Effect And Its Mechanism Of Fra-1Gene Silence On Gastric Cancer MGC-803Cell Invasion And Migration

ObjectiveSilence Fra-1gene to see its effect and mechanism on gastric cancer MGC-803cellinvasion and migrationMethodsWe used RNA interference to silence Fra-1gene.And the expression of mRNAof Erk、uPA、Fra-1、MMP-9、uPAR、TIMP-3was tested byRT-PCR; the expression ofprotein of Erk、Fra-1、uPA、uPAR、MMP-9、TIMP-3was determined by Western-Blotassay.We detected cell invasion and migration capability using Trans-well chamber methodand scratch test.Results1. Preliminary research has confirmed that the transfection of targeted Fra-1SiRNAinto gastric cancer MGC-803cells can silence the expression of Fra-1factor2. Trans-well chamber method and scratch assay showed that compared with other controlgroups, cell invasiveness and migration capability was significantly lower after Fra-1factor silence.3. RT-PCR result showed that mRNA expression of ERK, uPA, MMP-9wasreductied in MGC-803cells after silencing Fra-1factor（P <0.05).However themRNA expression of TIMP-3was incresed（P <0.05), and there was no significant change in uPAR mRNAexpression (P>0.05).4. Western-blot result showed that protain expression of ERK, uPA, MMP-9was reducedin MGC-803cells after silencing Fra-1facto（rP <0.05).However the protain expression ofTIMP-3was incresed（P <0.05), and there was no significant change in uPAR protainexpression (P>0.05).Conclusions1. Fra-1gene silence can significantly inhibit invasiveness and migration capability ofgastric cancer MGC803cells2. After Fra-1gene silence, mRNA and protein expression levels of gastric cancer invasion and metastasis-related factors: Erk、uPA、MMP-9were reduced,confirming that Fra-1gene may be the target gene in ERK/Fra-1/uPA/MMPs pathway on regulating the invasionand metastasis of gastric cancer.