| Objiective: To study the Effect of ESAT-6on expression ofinterleukin-17in γ T cells and explore its mechanism of signaling pathway.Methods: Peripheral blood mononuclear cells (PBMC) were collectedfrom health human blood with the ficoll density gradient centrifugationmethods, and the T cells were gained by the nylon wool column method,and then the γ T cells were separated from the T cells with Flow cytometry.The zoledronic acid (ZA) and interleukin-2(IL-2) were used to stimulateand expand the γ T cells. The γ T cells were treated and stimulated byESAT-6after12days cultured, and the inhibitor of STAT3signalingpathway (S3I-201) was administered, besides control groups withoutESAT-6and stimulator were established. The mRNA of IL-17and STAT3were determined by methods of PCR, and the expression of STAT3proteinwas analyzed by the method of Western blot, and then the change of IL-17was tested by ELISA. Results: ESAT-6could increased the expression of IL-17and STAT3(P<0.01), and the inhibitor of STAT3(S3I-201) could decreased expressionof IL-17(p<0.01) in γ T cells.Conclusion: ESAT-6can enhance the expression of IL-17producedby γ T cells, and the mechanism of which maybe concerned with STAT3signaling pathway. |
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