Protective Effect Of HMGB1Gene Silence On Astrocytes After Oxygen-glucose Deprivation/Reoxygenation In Vitro

Objective To investigate the protective effect of high mobility groupbox（lHMGBl）gene silence on oxygen-glucose deprivation/reoxygenation（OGD/R）-induced injury in astrocytes in vitro．Methods Astrocytes were divided into control group，OGD/R groupand HMGB1siRNA group．Astrocytes in OGD/R group were treated withOGD for2，4，and6h respectively，and then reoxygenated for24h．InHMGB1siRNA group， astrocytes that transfected with HMGB1smallinterference RNA（siRNA） lentivirus-vector were treated with OGD6h，and then reoxygenated for24h．The protein of normal astrocytestransfected with HMGB1small interference RNA （siRNA）lentivirus-vector were inspected by Western-blot.After24hours’reoxygenation，The mRNA and protein expression of HMGB1in theastrocytes were detected by RT-PCR and ELISA method.The cell injuryand survival rate were inspected by LDH activity（LDH％） and MTTassay． Results Compared astrocytes without transfected with HMGB1small interference RNA（siRNA）lentivirus-vector，The protein expression of HMGB1can be suppressed by small interference RNA. Compared withnormal group，as the OGD time prolonged，the mRNA and proteinexpression of HMGB1were increased gradually（P<0.05） after OGD/R，which were further increase with time elapse. OGD/R resulted insignificant injuries with time prolonged，the LDH％was increased（P<0.05） and the survival rate was decreased severely（P<0.05）．Compared with OGD/R group，cell injury in HMGB1siRNAgroup was remarkably decreased，as survival rate was raised significantly.Conclusions Expression of HMGB1in astrocytes can be inhibitedby small interference RNA，over-expression of HMGB1might be animportant factor in OGD/R-induced cell injury．...