Effects Of Electro-Acupuncture On Ampk In Skeletal Muscle Of Obese Rats

Objective:To observe the effects of electro-acupuncture(EA) at ST36and ST45treating foodborne obese rats and influence on the expression of skeletal muscle Amp-activated protein kinase,Acetly-CoA carboxylase and carnitine palmitoyl transferase-1.Method:fat group30to high-fat diet.After the high-fat diet,induced fat three months,with10%of body weight than ordinary standard diet group were obese,defined as foodborne obese rats.The successful modeling obese rats were randomly divided into EA group and model group,each group has seven.Normal control group rats were randomly selected seven.EA group:acupuncture ST36and ST45, electro-acupuncture device is connected, the frequency2/15Hz, the intensity of1mA, time15min, alternating left and right side every other day rotation acupoints.Model group: Like bondage with electro-acupuncture group, but not electro-acupuncture;Normal group:without any treating.Treatment six days a week to rest one day, a total of4weeks of treatment.During treatment for each group of rats are fed with normal diet.Comparing rats’ body’s weight,body fat,TC,TQLDL-C.Expression of skeletal muscle AMPKa-mRNA, ACC-mRNA,CPT-1-mRNA were mersured by real-time quantity PCR.Result:Comparing electro-acupuncture groups with model group, the differences of each index have notable statistical significance (p<0.01), and comparing with normal group, there is no statistical significance (p>0.05). Between three electro-acupuncture groups, there exist differences, which is not so distinguished.Conclusion:electro-acupuncture at ST36and ST45can reduce body’s weight,body fat,blood lipids. increase the expression of P AMPKα/AMPKα、P ACC/ACC、CPT-1/βActin in skeletal muscle.Inhibiting the synthesis of fat and promote fat decomposition.