Tumor Pathology And DCE-MRI In The Comparative Study Of Colorectal Carcinoma Angiogenesis In Nude Mice With Evaluation

Objective:To establish the model of colon cancer in nude micesubcutaneous tumor, analysis of tumor pathology and dynamic contrast-enhanced scan (DCE-MRI) with pharmacokinetic parameters obtainedcorrelation reflect vascular permeability, practical advantages incomparison of two methods for evaluation of tumor angiogenesis.Methods:30nude mice model of colon cancer in four consecutive days of routine MRI and dynamic contrast enhanced MRI scan, dynamic observation parameters reflecting vascular function, contrast agent volume transfer constant Ktrans, reflux rate constant Kep, the vascular spacevolume fraction Vp, extravascular extracellular space volume Ve, signal maximum rising slope MSI, respectively in the third day, fourth days after the end of each magnetic scanning15rabbits killed nude mice, thepathological immunohisto chemistry, microvessel density (microvesseldensity, MVD) count, vascular endothelial growth factor (Vascularendothelial growth factor, VEGF) score, proliferating cell nuclear antigen (Proliferating cell Nuclear antigen, PCNA) detection, analysis nuclear magnetic resonance parameters and colon cancer pathology and immunohistochemistry index of correlation. Results:From the observation time of the first day of the fourth day, Ktrans, Kep and MSI value increased gradually, comparison among various time statistical difference (P<0.05),there were no statistical differences inVe, Vp each time segment (P>0.05).Ktrans, Kep and MSI three parameters respectively have positive correlation with the MVD count and VEGF score, are positively related to Ktrans,MSI and PCNA score. A positive correlation between MVD count, VEGF score and PCNA score of two two. Conclusion:The pathological indicators and imaging indexes have consistency in the evaluation of tumor angiogenesis, DCE-MRI technology can be used as a marker to evaluate imaging of tumor angiogenesis.