Pancreatic Tissue TLR4and Inflammatory Factor Expression In High-fat Diet Insulin Resistance In Mice

Objective:Insulin resistance in obesity is associated with a chronic systemic low-grade inflammation. The study aims to explore the relationship between the TLR4/NF-κB signaling pathway and high-fat diet induced insulin resistance in mice pan creatic tissue. Methods:C57BL/6mice(80) were randomly divided into two groups, which were feeded with normal diet and high fat diet respectively, and then rand omly divided into eight groups according to the feeding days (0,3,7,11,14,21,42,84days respectively). The weight, fasting blood glucose and serum insulin levels wer e detected, and the pancreatic tissue was observed through tissue slice with HE. I mmunohistochemistry was used to detect the expression of inflammation factors sue h as TNF-a, IL-6so as to describe the formation and development of fat local inf lammation. Finally, the Western-blot method was adopted to detect proteins of TL R4/NF-κB. Results:The results showed that the mice insulin resistance model we re successfully created with high-fat diet, and high-fat diet induced the expression of TLR4mRNA and protein in pancreatic tissue. TLR4/NF-κB signaling pathway activation increased gradually with the seven days of high-fat diet given, and whic h reached a maximum level at the21st day. The islet cell increased TNF-a and I L-6expression. Conclusion:the high expression of TLR4/NF-κB in pancreatic tiss ue and the increasing of serum insulin is consistent. The high-fat diet can activated fat cells TLR4/NF-κB signaling pathways, and have the association with the insu lin resistance.