The Protective Effects Of Catalpol On High Glucose Induced Apoptosis In SH-SY5Y Cells

ObjectiveTo investigate the protective effects of catalpol on high glucose inducedapoptosis and its mechanism.MethodsThe cells were randomly divided into control group, apoptosis group,catalpol group, catalpol protected group and mannitol group. The apoptosis ofSH-SY5Y was induced by50mmol/L glucose for48hours, catalpol protectedgroup be composed of the different final concentration of catalpol (1,2,4mg/ml)and glucose50mmol/L were added together for48hours. The cell viability wasanalyzed by MTT assay; the change of nuclear was assayed by Hoechst33258;the apoptosis rate was measured by flow cytometry.The level of8-OHDG wasdetermined by enzyme-linked immunosorbent assay.ResultsCompared with control group, the apoptosis group’s cell viability wasdecreased; the early apoptosis rate and the content of8-OHDG were increased;karyopyknosis, split and apoptosis were found by Hoechst33258dyeing.Compared with apoptosis group group, the cell viability of catalpol (1,2,4mg/ml) protect groups were obviously increased; the early apoptosis rate andthe level of8-OHDG were significantly decreased; the nuclear shape wasclearly ameliorated. ConclusionsThe results show that Catalpol has protective effect on high glucoseinduced apoptosis; the mechanism may be related to its antioxidation.