Expression And Effect Of ABCE1Gene In Esophageal Squamous Cell Carcinoma

ObjectiveTo investigate the differential expression of ABCE1gene and RNaseLbetween esophageal squamous cell carcinoma and adjacent tissues, andfurther clarify the ABCE1gene in proliferation, invasion, migration, apoptosis ofcells of esophageal squamous cell carcinoma and discuss its mechanism ofaction preliminarily.MethodsDifferential expression of ABCE1and RNaseL between esophagealsquamous cell carcinoma and adjacent tissues were detected by Western blotassay.ABCE1-SiRNA were transfected into Eca109cell line. After transfection,the efficiency of transfection was observed under a fluorescence microscope.Gene silencing was examined by Western blot. RNaseL was examined byWestern blot. The proliferation, invasion, migration and apoptosis ability of thecells was assayed by MTT, transwell,scratch and flow cytometry.Results1, ABCE1gene was low expressed in esophageal adjacent tissues andwas high expressed in esophageal squamous cell carcinoma.The expression ofABCE1gene was higher in esophageal squamous cell carcinoma tissues ofdifferent degree of differentiation than in adjacent tissues（*P<0.05）.RNaseLwas high expressed in esophageal adjacent tissues,was low expressed inesophageal squamous cell carcinoma（#P<0.05）.2, After transfected with ABCE1-SiRNA, green fluorescence could be seenin esophageal cancer cells.3, Gene silencing was confirmed by western blot (*P<0.05) and validated ofthe RNaseL pathway conjecture. 4, MTT tests showed that proliferation ability of the cells which transfectedwith ABCE1-SiRNA was much lower than the negative control group and blankcontrol group (*P<0.05).5, Transwell tests revealed that the cells penetrating the artificial basementmembrane in ABCE1-SiRNA were fewer than those in the negative controlgroup and blank control group (*P<0.05).6, Cells which transfected with ABCE1-SiRNA migrated obviously sloweddown,compairing with the negative control group and blank controlgroup(#P<0.05).7, The apoptosis rate detecting by flow cytometry showed that theapoptosis rate in cells transfected with ABCE1-siRNA were significantly higherthan the negative control group and blank control group cells (*P<0.05).Conclusions1, Expression of ABCE1may be closely associated with the occurrenceand development process of esophageal squamous cell carcinoma.ABCE1gene was low expressed in esophageal adjacent tissues and was highexpressed in esophageal squamous cell carcinoma.Mechanism of the ABCE1gene effecting on human esophageal carcinoma cells by inhibiting the activity ofRNase L, blocking the pathway of2-5A/RNase L, and then influnting thebiological behavior of the tumor.2, ABCE1gene is involved in the biological behavior of proliferation,invasion, migration and apoptosis of esophageal squamous cell carcinomacells.ABCE1gene inhibit apoptosis of esophageal squamous cell carcinomacells by inhibiting the activity of RNase L and blocking the pathway of2-5A/RNase L.The activity and invasion of esophageal squamous cellcarcinoma cell was enhanced and cell migration and proliferation becomefaster.