Analysis Of Difference In Intestinal Flora Of Uygur And Han Ethnic Chinese Patients With Ulcerative Colitis

Objective:To identify correlations between intestinal flora and ulcerative colitis, we analyzed differences in amounts of Bacteroides, Fusobacterium, Clostridium, Bifidobacterium spp, and Faecalibacterium prausnitzii in the intestinal microflora of Uygur and Han ethnic Chinese patients with ulcerative colitis (UC) and healthy controls.Methods:Gene-specific primers for16S rDNA of Bacteroides, Fusobacterium, Clostridium, Bifidobacterium spp, and Faecalibacterium prausnitzii were designed. Feces of UC patients and healthy controls were collected. Bacterial genomic DNA extracted from fecal samples was used for quantitative analysis by real-time fluorescence quantitative polymerase chain reaction (PCR) to analyze the amounts of each of the5bacteria. Results:No significant differences in any bacteria were observed between Uygur and Han populations for both healthy controls and UC patients. In the Uygur population, UC patients exhibited a significantly increased Bacteroides population and significantly decreased Clostridium, Bifidobacterium spp, and Faecalibacterium prausnitzii populations. The quantity of Fusobacterium did not differ significantly between the2groups. Compared with healthy controls, the amount of Bacteroides significantly increased, while the amounts of Clostridium, Bifidobacterium spp, and Faecalibacterium prausnitzii significantly decreased when the Uygur and Han UC patient populations were combined. Moreover, similar to Uygur UC patients alone, the amount of Fusobacterium did not differ significantly between Uygur and Han UC patients and healthy controls. In the UC population,compared with the patients in remission,the patients in activity exhibited a significantly increased Bacteroides population and significantly decreased Clostridium, Fusobacterium,Bifidobacterium spp, and Faecalibacterium prausnitzii populations. Conclusions:Variations in microbial loads may be associated with the occurrence and activity of UC in Uygur and Han populations; however, these variations were not associated with ethnic differences. Further studies are required to fully elucidate the characteristics of microbial loads in UC patient populations.