Correlation Of MIF Polymorphism With Idiopathic Orbital Inflammatory Pseudotumor Etc. Diseases

Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokinewhich is closely related to the important pathophysiological process, suchas inflammation, immune responses, glucose metabolism, cell proliferation andapoptosis. MIF plays an important role in the pathogenesis of immune diseases.Idiopathic orbital inflammatory pseudotumor (IOIP), orbital cavernoushemangioma (OCH), Mikulicz disease and intraocular lymphoma are common eyediseases. As the etiology of these diseases is not clear, treatment remains a challenge inophthalmology. To study the pathogenesis of these diseases becomes extremely urgent.In this study, we collect clinical eye samples and study MIF gene polymorphismwith eye diseases including IOIP etc. We have finished the following work as follows:1. Set up clinical sample database. Clinical eye diseases were collected about270cases including blood and tissue. The blood was centrifuged to separate plasma andblood cell and DNA was extracted from blood. Part of the tissue was made to paraffinsection and HE staining. Samples were classified according to the type of tissue cells.Other tissues were used to extract protein and RNA. The establishment of clinicalsample database could lay a solid foundation of eye diseases study with importantsignificance.2. The method of ELISA was applied to study MIF expression of the plasma. TheMIF concentration of IOIP, OCH, Mikulicz disease and intraocular lymphoma samples’plasma were28370±3466pg/mL,30060±4785pg/mL,40820±4445pg/mL and49190±6041pg/mL, respectively, which were apparently higher than the controlsamples with1700±63pg/mL, P<0.0001. There were significant differencescomparing MIF concentration of IOIP samples’ plasma with those of Mikulicz diseaseand intraocular lymphoma samples, P<0.05; as well as comparing MIF concentrationof OCH samples’ plasma with that of intraocular lymphoma samples, P<0.05.3. The method of PCR and STR were used to analyze MIF gene promoter singlenucleotide polymorphism-173G/C and microsatellite polymorphism-794CATT5-7with small sample size. MIF polymorphism-173G/C is irrelevant to the susceptibilityof IOIP, OCH, Mikulicz disease and intraocular lymphoma, but allele C could be a cause of the increation of related diseases susceptibility. MIF polymorphism-794CATT5-7is irrelevant to the susceptibility of IOIP, OCH, Mikulicz disease andintraocular lymphoma, but allele CATT7could be a cause of the increation of relateddiseases susceptibility as well.In conclusion, this study indicated that MIF gene played an important role in thepathogenesis and treatment of IOIP, OCH, Mikulicz disease and intraocular lymphoma.