Clinical Studies On The IBS-D And Intestinal Mucosal Mast Cells Level

Objectives By comparisiong the change of mucosal mast cells number betweendiarrhea-predominant irritable bowel syndrome and normal control group, and analysingthe differences of the number of mast cells between in ileum, ileocecum and rectum-sigmoid junction among various groups. Providing new clues for the study of thepathogenesis of IBS.Methods1From January2012to June2013we choosed20numbers of IBS-D patientswho visited the Hebei United University gastroenterology clinic and inpatient departent.All of the patients followed the Rome Ⅲ diagnostic criteria and as the case group.While20numbers of no gastrointestinal symptoms in healthy volunteers as the control group.2All subjects need to signed the informed consent of endoscopy before colonoscopy. Webiopsied2pieces of colonic mucosal tissue form ileum, ileocecum and rectum-sigmoidjunction under endoscope by disposable biopsy forceps as histological specimens.Making slice after fixative fixed, and staining all the specimens with toluidine blue. Atthe end we counted the number of mast cells.3Statisticaled the basic data of all subjectsby the general questionnaire (homemade).4Finally, established a database withSPSS17.0software for statistical analysis, and all of the data for statistical analysing andprocessing. All statistical data analysis were performed after normality testing andhomogeneity of variance testing. Measurement data using t test (approximate t test), andcount data by chi-square test. P<0.05was considered statistically significant.Results1The number of colonic mucosal mast cells in case group is significantlyhigher than those in the control group(23.45±8.27vs6.09±1.23,P<0.05), the difference isstatistically significant.2The numbers of3parts mast cells in case group are higherthan the control group:terminal ileum(21.49±5.62vs6.27±0.70, P<0.05), ileocecum(31.02±5.38vs6.93±1.49, P<0.05), rectum-sigmoid junction(17.82±7.38vs5.07±0.34,P<0.05), the differences are statistically significant.3The number of mast cells in casesgroup of the ileocecum is higher than those in terminal ileum and rectum-sigmoidjunction (31.02±5.38vs21.49±5.62vs17.82±7.38, P﹤0.05), and the difference isstatistically significant.4The number of intestinal mucosal mast cells in case group offemale patients is higher than the number of male patients(25.47±7.56vs19.97±8.47, P<0.05), the difference is statistically significant.Conclusion1The number of mucosal mast cells with IBS-D patients is increasing.2The number of mucosal mast cells in different parts of the intestinal with IBS-D patientsare all increasing, especially in the ileocecum.Figure6; Table9; Reference100...