Inhibition Of SENP1/HIF-1α Pathway Attenuates Acute Lung Injury Induced By Intestinal Ischemia/Reperfusion

ObjectiveTo explore the initial mechanism regarding to inhibition of SENP1/HIF-1α pathwayattenuating acute lung injury induced by intestinal ischemia/reperfusion throughobserving the effects of acute lung injury induced by intestinal ischemia/reperfusionin SENP1+/-mice.MethodsCultivation and siRNA transfection of HCT-8cell; testing knockout efficiency ofSiHCT-8cell; testing SENP1mRNA, HIF-1α, Caspase-3and iNOS levels of the cellgven hypoxia/reoxygenation.Testing knockout efficiency of SENP1+/-mice; testing SENP1mRNA, HIF-1α,Caspase-3,and iNOS levels in ileal tissue of mice, also MPO acitivity, IL-6mRNAand IL-10mRNA levels in lung tissue of mice underwent intestinalischemia/reperfusion.ResultsSENP1mRNA levels of HCT-8underwent hypoxia/reoxygenation and WT micesubjected to intestinal ischemia/reperfusion were significantly increased comparedwith normal/control group; Decreased MPO, IL-6mRNA and IL-10mRNA levelswere found in lung of SENP1+/-mice subjected to SMAO relative to WT mice;HIF-1α, Caspase-3and iNOS levels were significantly decreased in SiHCT-8underwent hypoxia/reoxygenation compared with NsHCT-8; HIF-1α, Caspase-3andiNOS levels were significantly decreased in ileum of SENP1+/-mice subjected toSMAO relative to WT mice.ConclusionInhibition of SENP1/HIF-1α pathway attenuates acute lung injury induced byintestinal ischemia/reperfusion.