The Toxicity Study Of Mesoporous Silica Nanoparticles SBA-15on Human Umbilical Vein Endothelial Cell

Objective: Along with the development of nanotechnology, the application ofnanomaterials is widening, and many nanoparticles have gone into the human survivalspace. Meanwhile, there will be more and more people exposed to nanomaterials. Thebiological safety of nanomaterials has attracted widespread attention for the uniquesmall size effect, surface effect and macroscopic quantum tunneling effect, and soon.While it come into the body, it may trigger a special biological effects. Because ofthe excellent structure performance of mesoporous silica nanoparticles (MSN), whichare now widely used in the study of drμg carrier. Once in clinical use, human bodywill be exposed to them more directly, and their biological effects need moreattention.Vascular endothelial cells is the first defense between bureaucratic andhemal wall deep, and previous studies found that endothelial injury was theprerequisite of many cardiovascular diseases.This study choose mesoporous silicananoparticles SBA-15and human umbilical vein endothelial cells(HUVEC) as thestudy objects to explore the toxic effects and mechanism of MSN on thecardiovascular.Methods:HUVEC were exposed to the RPMI1640medium containing a finalconcentration of0(solvent control),25,50,100and200μg/ml of SBA-15and100μg/ml micron silica to be cultured for24h. Then detect the inhibition on HUVECproliferation of SBA-15with methyl thiazolyl tetrazolium(MTT) assay. Detect theapoptosis effect of SBA-15on HUVEC by adopting double staining method withfluorescein isothiocyanate-Annexin V（Annexin V-FITC）and propidium iodide (PI), and detect the change of ROS level in HUVEC by using dichlorofluoresceindiacetate(DCFH-DA) probe method. Use quantitative real-time polymerase chainreaction(qRT-PCR) to detect the changes of cytokines CRP, MCP-1, ICAM-1andVCAM-1gene mRNA expression level under the condition of SBA-15. And theprotein expression level of ICAM-1and VCAM-1gene were detected by WesternBlot technology.Results:Compared with the solvent control group, different concentration ofSBA-15had significant inhibitory effect on HUVEC proliferation，the difference wasstatistically significant (P<0.05), and with the increase of SBA-15concentration, andthe cell vitality significantly declined. Compared with blank control group, theHUVEC apoptosis rates of the groups contaminated by50,100and200μg/mlSBA-15solutions increase，the difference was statistically significant (P<0.05), thecells’ ROS level rises with the increase of SBA-15concentration.With the increase ofSBA-15concentration, cytokines CRP, MCP-1, ICAM-1and VCAM-1geneexpression level of HUVEC increased，the difference was statistically significant(P<0.05).Conclusion:SBA-15has toxic effects on HUVEC, which can reduce the cellvitality; Cell apoptosis and necrosis are the coexist cell death way under the conditionof SBA-15; SBA-15may cause atherosclerosis disease; Oxidative damage andinflammatory may be the important poisoning mechanism of SAB-15to HUVEC.