The Electrotaxis Of Mice Melanoma Cells On Different Matrix And PH

Recent studies show that electric signal could guide the directional migration ofthe tumor cell and regulated by various factors, such as growth factors、extracellularmatrix and acid and alkali environment, etc. So far, the electrotaxis of tumor cell isstill poorly understood.In this study, using mice skin melanoma cell (B16-F10) as a model, we studiedthe electric fields guided cell migration, for the purposed of providing new ideas forthe future clinical diagnosis and treatment of cancer electrically.Research content:1. Melanoma cells migration on three different type of substrate in the electricfield, the smooth and biomimetic substrate; using250mV/mm electric field strengthfor2hours, then quantify cell migration as directedness, track speed, and persistence.2. Melanoma cells migration in different electric field strengths, compared withno electric field, constant stimulation respectively using three different electric fieldstrengths (100mV/mm,250mV/mm,350mV/mm) for2hours, then quantify cellmigration as directedness, track speed, and persistence.3. Melanoma cells migration in different pH (6.6,7.0and7.4), using100mV/mm electric field strength for2hours, then quantify cell migration asdirectedness, track speed, and persistence.The results showed that:1. Biomimetic membrane has the similar surface structure with animals’epithelial basement membrane. In the electric field, the cells show obvious migrationto the cathode of electric field on three different type of substrate, there is nostatistical differences between smooth and biomimetic membrane substrate, but thereare significant differences in the speed and persistence data, significant difference intrack speed (P<0.01), significant difference in persistence (P<0.05).2. The melanoma cells migrate extremely slowly in the absence of appliedelectric field, displacement is less than5microns in2h, and randomly, no fixeddirection. But the cells have a obvious directional migration in the electric field, the migration speed, direction and persistence show an enhanced trend with the increaseof electric field strengths and significant difference (P<0.01).3. Melanoma cells also show significant difference of eletrotaxis in different acidenvironment. Directedness, track speed, and persistence go up with the decrease ofpH (P<0.01).