| Objective: Sound-light-electric multiple stress stimulation was used to establish therat model in premature ovarian failure. To observe the effect of DingJingTang ongastric antral smooth muscle contraction, gastric slow-wave, gastric emptying,smallintestinal propulsion rates, gastric acid, pepsin activity and gastric ICC. Discussionthe effect of DingJingTang on gastrointestinal function of premature ovarian failurerat mode and its mechanism.Methods:1. Establish the premature ovarian failure rat model. Buy a batch of SPF SDfemale rats, Weight180-220g, Adaptive feed after5d, Vaginal smear regularly everyday with a microscope observation, Choose have two consecutive normal estrus ratsused to build model experiment, and the rest out.After screening the rats wererandomly divided into normal group and model group, each group of20, a total of40.The main stimulation method: Sound-light-electric multiple stress stimulation (Soundand light stimulation10s at the same time, add feet shock.Sound-light stimulation tostop after60s. Electric shock plantar maintain5s, a total of75s). Additional stressmethod:Fasting for24h. Ban water for24h. Electric shock a foot.4℃cold waterswim5min. All night lighting. The clip tail1min.45℃oven heat drying5min.Random every day to give a stimulus. Continuous building20d. By observing thesigns of rats, estrous cycle, tectology and the level of FSH and E2changes todetermine whether a building success.2. The effect of DingJingTang on gastric antral smooth muscle contraction.20female rats were randomly divided into normal group and modal group. MP150biopac systems was used to collecting contraction curves of gastric antral smoothmuscle in the each thermostatic perfusion slot. To observe the gastric smooth musclecontraction.The56premature ovarian failure rat model were randomly divided into the whole decoction group, main drugs group, no major drugs group, shugan groupand bushen group. Give each group rats concentration of high, medium and low threecorresponding drugs. Confirm the optimum concentration and the best formula.Follow-up experiments are using this formula.3. The effect of DingJingTang on gastric acid and pepsin activity.30femalerats were randomly divided into normal group, model group, medication group. Fromthe first day, normal group and model group to fill the stomach physiological saline,To medicine group to fill the stomach DingJingTang, Indexes such as frequency andpeak value of gastric slow-wave were recorded using multi-channel physiologicalrecorder MP150(BIOPAC, US) and AcqKnowledge Software.4. The effect of DingJingTang on gastric emptying,small intestinalpropulsion rates.30female rats were randomly divided into normal group, modelgroup, medication group. From the first day, normal group and model group to fillthe stomach physiological saline, To medicine group to fill the stomach DingJingTang,Continuous building20d, Fasting rats (can’t help but water, medicine)24h, normalgroup and model group to fill the stomach physiological saline,.To medicine group tofill the stomach DingJingTang. After30min to fill2%dextran blue2000solution.After20min neck push dislocated kill animals. Determination of gastric emptyingand small intestinal propulsion rates.5. The effect of DingJingTang on gastric acid and pepsin activity.30femalerats were randomly divided into normal group, model group, medication group. Fromthe first day, normal group and model group to fill the stomach physiological saline,To medicine group to fill the stomach DingJingTang, Continuous building20d,Fasting rats (can’t help but water, medicine)24h, normal group and model group tofill the stomach physiological saline,.To medicine group to fill the stomachDingJingTang. After1h, With urethane anesthetized rats and in abdominal incision.Pyloric ligation and duodenal junction. To observe the gastric acid and pepsinactivity.6. The ICC ultrastructure observation. Open the rat gastric cavity, take a stomach tissue. Put gastric specimens with cool Krebs fluid container. Removal ofgastric contents and do a series of processing. Under the transmission electronmicroscope observation groups of ICC ultrastructure of rat stomach.Results:1. Observing the changes of rat signs, estrous cycle, visceral weight andmorphology. It was obvious difference between normal and model group.Compared with normal group, ovarian tissue morphology of model group wasobviously damaged. FSH level significantly increases(P<0.01), while the E2levelsignificantly decreases(P<0.01).2. Compared with prior administration, the gastric antral smooth musclecontraction significantly increases both in high, medium and low concentration levelsof quangfang group and no-main drug group(P<0.01or P<0.05), while main druggroup, bushen group and shugan group sinificantly increases in high or mediumconcentration levels (P<0.05). Compared with quangfang group, shrinkage ofcalcium-free group and atropine group significantly decreases(P<0.01or P<0.05).3. Frequency and peak value of gastric slow-wave in rats of DingJingTanggroup increased significantly (P<0.01). DingJingTang can inhibit model of rat gastricelectrical slow wave rhythm disorder, promote gastric smooth muscle contraction.4. Model group gastric emptying and small intestinal propulsion ratessignificantly lower than normal group and drug group (P<0.01). DingJingTang canpromote ovarian premature failure model rat gastric emptying and small intestinalpropulsion rates.5. Model group content gastric acid and pepsin activity was significantlylower than normal group and treatment group (P<0.01). DingJingTang can promotethe secretion of gastric acid and pepsin activity.6. Compared with the normal group, The number of ICC overall modelgroup rats stomach is not much, ultrastructure is fuzzy, the organelles decreasedsignificantly, dissolved, cytoplasm, mitochondria, endoplasmic reticulum obviouslyexpansion decreased significantly, swelling, etc. After observation to drug group,Found DingJingTang on ovarian premature model rats stomach ICC has repair effect. Conclusion:1. It was feasible that the rat models of premature ovarian failure were setup by composite mental stimulation.2. The effect of dingjing tang and its separations is obvious, which relatedto calciumions and muscarinic receptor.3. DingJingTang can inhibit model of rat gastric electrical slow waverhythm disorder, promote gastric smooth muscle contraction.4. DingJingTang can promote ovarian premature failure model rat gastricemptying,small intestinal propulsion rates and enhance gastrointestinal dynamics.5. DingJingTang can promote the secretion of gastric acid and pepsinactivity.6. DingJingTang has repair effect on ovarian premature model ratsstomach ICC. |
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