UVA On The Damage Of Skin Tissue Engineering Inhibition Of Schizanthus B

Objectives To explore the Sanhedrin B against UVA damage and protectionmechanisms of tissue engineering skin.Methods1In Nitro immortalized cutin of choose and employ persons to form cells andfibroblasts silk fibroin modified polyamide composite non-woven creatures of eathpreparation of the epidermis,dermis tissue engineering skin model, the vaccination of1、3、5、7、9d to determination of determined cell growth on the stent is compared withthe blank group.2UVA damage skin tissue engineering design of experiment is dividedinto5group: blank group, the UVA irradiation different energy2、5、8、11J group.3Sanhedrin B drug protection experiment design is divided into six groups:UVAirradiation, the UVA irradiation+different concentrations of Sanhedrin B element0.01、0.1、1、10、100μmol/L group.4determination of Sanhedrin B element of UVA damagetissuey engineering skin SOD、NO、GSH、LDH content changes.Results1Immortalized cutin cells and fibroblasts with silk fibroin modified polyamidenon-woven creatures of successful compound growth on the surface of the gas and liquid.2According to the radiation intensity and the cell survival rate,the selected irradiationintensity of30mJ/cm2or5J UVA damage tissue engineering skin model established.3To choose the best protest the Sanhedrin B element concentration of0.01μmol/L、0.1μmol/L.4The results show that by enzyme chemistry method b element can increaseantioxidant enzymes in UVA damage to tissue engineering skin the content of SOD andGSH, reduce the content of NO and LDH.Conclusions Sanhedrin B can resist UVA to tissue engineering skin in Nitro ofoxidative damage effectively, Sanhedrin B development become a kind of new, highlyeffective UV protection products just around the corner.