Ningxia Fruitless Lycium Sprout Extracts On The Natural Aging Mice Studying On The Anti-aging Effects

Objective Aging is increased with age, each organ system in the body are degenerativechanges occur, is a comprehensive reflection of physiological, pathological and biochemicalreaction. Degenerative brain aging related change is a normal physiological phenomenon ofbrain growth mature aging process, the decline in learning and memory ability are the mainclinical manifestations of brain aging of central nervous system degeneration. The fruit ofChinese wolfberry is a traditional Chinese herbal medicine, Chinese medicine spleen and lung,kidney nourishing Yin drugs, has a function of delaying senility. Seedless medlar bud ismedlar new variety, content of bioactive substances in the extract was significantly higherthan that of ordinary Chinese wolfberry leaf, fruit and other plant. The purpose of this paper isto observe the Ningxia fruitless lycium sprout extracts (FLE) on the natural aging micecognitive function, brain tissue superoxide dis mutase (SOD), glutathione peroxidase (GSH-PX) activity, paraformaldehyde (MDA) content and the effect of expression of FOXO1, β-catenin, and provide laboratory evidence for the discussion of the possible anti-aging effectand mechanism.Methods15months of age, the natural aging KM mice were divided into control groupand old aged FLE low dose group (FLE1group), group of elderly patients with FLE (groupFLE2), aged FLE in high dose group (FLE3group),20rats in each group; the other20onlyMarch old KM mice as young control group. FLE1group, FLE2group, FLE3group weretreated with mass/volume percentage of5%,10%,20%FLE gavage,0.2ml/10g· d, agedcontrol group and young control group normal saline,8weeks in a row. Evaluation of effectof learning and memory ability of FLE on the changes of natural aging mice in the Morris water maze; Determination of brain tissue superoxide dis mutase of xanthine oxidase (SOD)activity, brain tissue glutathione peroxidase DNTB method (GSH-PX) activity, brain tissuelevels of MDA thio barbituric acid method; application of Nissl staining was used to observethe influence of FLE on the natural aging mice neurons of hippocampal CA2area density;immunofluorescence was used to detect FLE back to the influence of Burd labeled cells onthe synthesis of natural aging mice dentate; the expression of PCR was detected in braintissue of mice with FOXO1, β-catenae m RNA expression; immunohistochemistry and West-blot detection in brain tissue of mice with FOXO1,β-catenin protein.Results The use of different concentrations of FLE in natural aging mice after8weeks,the Ningsia no high dose boxthorn bud (FLE3) group, no dose boxthorn bud (FLE2) groupcan improve the natural aging mice and age related changes of behavior, improve the naturalaging mice learning memory ability, improve, decrease in hippocampal CA2region neurondensity due to aging, decrease of Nissl body, improve brain cells in the dentate gyrus ofsynthetic differentiation rate; improve the natural aging mice brain tissue superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) activity, paraformaldehyde (MDA) content,trend effect was positively correlated with FLE concentrations.; PCR assay showed that FLE3group, FLE2in brain tissue of mice FOXO1, β-catenae and increased the expression of mRNA immunohistochemistry and West-blot assay showed that FLE3group, expression inbrain tissue of mice in FLE2group of FOXO1, β-catenin protein increased, and consistentwith the ability and seedless medlar bud oxidative stress.Conclusions The application of Ningxia seedless medlar bud extract can obviouslyimprove the learning and memory ability of natural aging mice, improve brain degenerativechanges associated with age, based its anti-aging effect probably by increasing the naturalaging mice brain tissue antioxidants such as superoxide dismutase (SOD), glutathioneperoxidase (GSH-PX) activity, reduce the formation of MDA, FOXO1, up regulation of theexpression of β-catenin, improve the brain cells in the dentate gyrus and differentiation rate of synthesis, a large number of free radicals and improve brain tissue due to aging and naturalaging mice caused accumulation of neurons in hippocampal CA2area density causes adecrease, and then improve the ability of learning and memory in natural aging mice.