Expression And Clinical Siganificance Of MAGE-A9 And MAGE-A11 Protein In Laryngeal Squamous Cell Carcinoma

Objective:The laryngeal carcinoma is one of the malignant neoplasms in otolaryngology surgery,which accouts for 1%-5% of all neoplasms.In recent years,the incidence has increased significantly.Until now,nobody can expain the pathogenesis of the laryngeal carcinoma,but some research results suggest that multiple factors contribute to the occurrence,development and metastasis of the carcinoma.To our knowledge,surgery,radiotherapy and chemotherapy are accepted therapeutic methods.To date, the therapeutic effect and the prognosis of laryngeal carcinoma is still not ideal.Now,a rising tumor therapy--tumor immunotherapy,which is regarded as the fourth therapeutic method,is coming into our horizon.Its feature is to stimulate and strengthen body’s immunity,to kill targeted composition in the tumor cells.In the process,identification of appropriate target antigens is the most crucial step.Cancer/testis antigens(CTAs) is expressed in a variety of malignant tumors, but not in normal adult tissues except for testis and placenta.The testis tissue is regarded as immune privilege organ.These features make CTAs just the potential ideal target antigens for tumor immunotherapy.The subgroup of CTAs,melanoma-associated antigens family A(MAGE-A),is of higher tumor specificity.Therefore,MAGE-A becomes research focus.In the review,we build up the research about the expression of MAGE-A9 and MAGE-A11 proteins, and analyze their correlation with clinical parameters, which provides theory for the immunotherapy.Methods:1 Immunohistochemistry(streptavidin-perosidase method) was employed to measure expression of MAGE-A9,MAGE-A11 proteins in 48 specimens of laryngeal squamous cell carcinoma(LSCC) tissues,20 specimens of adjacent non-neoplastic tissues and 6 specimens of normal adult testis tissues,and expression of ki67 protein in 48 specimens of LSCC tissues and 20 specimens of adjacent non-neoplastic tissues.2 Clinical data were analyzed with the SPSS statistical software version SPSS13.0,P<0.05 indicating statistical significant difference,P<0.01 indicating great significant difference.Results: 1 Expression of MAGE-9 and MAG-A11 proteins in the normal human testis tissues.In the normal testis tissues, MAGE-A9 and MAGE-A11 proteins were observed in both cytoplasm and nuclei of the primary spermatocytes and spermatogonia. 2 Expression of MAGE-A9 and MAGE-A11 proteins in both LSCC tissues and adjacent non-neoplastic tissues 2.1 Positive rate of MAGE-A9 in LSCC tissues and adjacent non-neoplastic tissues were 45.8%(22/48)versus 0%(0/20).There was great significant difference between the two groups(P<0.01).MAGE-A9 protein was found in cytoplasm,but occasionally also observed in nuclei of LSCC. 2.2 Positive rate of MAGE-A11 in LSCC tissues and adjacent non-neoplastic tissues were 52.1%(25/48) and 0%(0/20).There was great significant difference in the positive rate of MAGE-A11 protein between LSCC tissues and adjacent non-neoplastic tissues(P<0.01).MAGE-A11 protein was found in cytoplasm,occasionally also observed in nuclei of LSCC. 3 The relationship between expression of MAGE-A9,MAGE-A11 proteins and clinical parameters 3.1 No association was observed between pathological degree,clinical stage,clinical classification,age,gender,lymph node metastasis,smoking,tumor size and the expression levels of MAGE-A9 protein(P>0.05). 3.2 Expression of MAGE-A11 was correlated with clinical stage(P<0.01),lymph node metastasis(P<0.05) and tumor size(P<0.05),but not with pathological degree,clinical classification,age,gender and smoking(P>0.05). 4 The correlation between expression of MAGE-A9,MAGE-A11 proteins and ki67 LI.In LSCC tissues,MAGE-A9 protein(P<0.01) and MAGE-A11 protein(P<0.05) had significant correlation with ki67 LI.Conclusions:1 MAGE-A9 and MAGE-A11 were not observed in adjacent non-neoplastic tissues,but observed in LSCC tissues.The positive rate of MAGE-A9 and MAGE-A11 proteins in LSCC tissue was significantly higher than that in adjacent non-neoplastic tissues.These data suggest that MAGE-A9 and MAGE-A11 may paticipate in the occurrence of LSCC,and indicate that they are of relative tumor specifity.2 The positive rate of MAGE-A11 protein expression in LSCC tissues had relationship with clinical stage,lymph node metastasis and tumor size.It suggests that MAGE-A11 may participate in the progress and metastasis of LSCC tissue.3 In LSCC tissue, MAGE-A9 and MAGE-A11 protein expression had positive correlation with ki67 LI.These data indicate that MAGE-A9 and MAGE-A11 are related to cell proliferation in LSCC.