Experimental Study Of Qi Shen Huan Wu Capsules Pharmacology Effect On Rat’s Sciatic Nerve Entrapment Damage

Objective: This paper intends to study Qi Shen Huan Wu Capsules drug orally in the application of rat sciatic nerve entrapment model, by changing the light microscope to observe the organizational structure of the sciatic nerve entrapment, gastrocnemius motor endplate staining and morphological changes and electron microscope gastrocnemius muscle endplate changes and sciatic nerve sections ultrastructural changes. Compared the differences between experimental group and control group in nerve repair and regeneration, in order to clear Qi Shen Huan Wu Capsules promote repair and regeneration of peripheral nerve function and effect of the location and the time window for integrative medicine treatment of peripheral nerve entrapment disorders that can provide theoretical and experimental evidence.Methods: Male Sprague-Dawley rats were 48, weighing 230 g ± 20 g, clean level.Sciatic forceps produced experimental rat model with 1.5% sodium pentobarbital(0.2ml / 100g) for intraperitoneal injection of anesthesia. After anesthesia successful, routine skin preparation, disinfection, shop towels, separate sciatic nerve, pears in the right gluteus gap shaped muscle at the lower edge of 0.8cm with a large hemostat clamp sciatic nerve, the pressure over three buckle, squeezed five seconds after the released 10 seconds, repeated three times to close the incision.After modeling by randomly divided into experimental group and the control group 24, sub-cage feeding. After the second day, modeling group was given medicines, oral administration of the experimental group Qi Shen Huan Wu Capsules Yaomo salt solution, 3g crude drug / ml, 1ml / 100 g of body weight, 1 times / day; the control group received normal saline 1ml / 100 g of body weight, 1 times / day. 1,2,4,8 weeks after the experimental and control groups were compared randomly from each of six.Contents: a light microscope: 1) light microscope sciatic nerve entrapment: myelinated fiber hyperplasia, myelin changes in axonal and vascular regeneration.2) gastrocnemius motor end plate light microscopy: gastrocnemius change shape; motor endplate staining and structural change. Observation2: 1) sciatic nerve entrapment : the changes of myelin, axons, Schwann cells,mitochondria, microtubules and other ultrastructure was observed.2) gastrocnemius electron microscopy: different periods on the motor end plate at the change sarcomere, axons, Schwann cells, primary folds, secondary folds, the impact of synaptic vesicles.Results: 1 Animals overall observation Normal diet of rats after modeling, limping, walking dragging step, incision healed well, No swelling, ulceration and infection, autophagy. One week after modeling, the right hind huddle, calf muscle two weeks with varying degrees of atrophy, limb function is limited. From the recovery situation, the two groups showed no difference in two weeks, two weeks later, the experimental group than the control group. 4 weeks followed Extension of time, gait and muscle gradually returned to normal.2 A general observation two weeks after modeling, the experimental group and control group had no significant differences in changes of entrapment of the nerve edema, entrapment at thinning the distal entrapment neuroma-like changes visible. Nerve damage caused atrophy of Gastrocnemius muscle atrophy dominant branch. At 4 weeks, nerve entrapment had been obvious edema, thickening of nerve entrapment, gastrocnemius thickening in the experimental group; control group entrapment nerve edema was reduced comparing with previous, but not as good as the experimental group was significantly changed, the gastrocnemius muscle thickening obvious. When two groups of eight weeks nerve entrapment edema disappeared, nerve entrapment at the significantly enlarged, closed to normal.3 light microscope 3.1 distal nerve entrapment After modeling two weeks, there was no significant changes, perineural a large number of inflammatory cell infiltration, there were a small number of myelinated fibers, myelin uneven thickness, myelin depigmentation obvious connective tissue between the beam significantly fewer blood vessels. At 4 weeks, when the number of myelinated fibers increased significantly, the experimental group increased the number of coarse fiber ratio, significantly thicker myelin, less myelin disintegration, no oval formed, connective tissue between the beam significantly reduce, vascular proliferation significantly, rich blood supply. The control group had an increase in the number of myelinated fibers, but had small myelinated fiber-based, thin myelin sheath, myelin disintegration obvious, visible oval formation, the connective tissue between the beam more vascular proliferation.At 8 weeks, when the number of myelinated fibers increased significantly, the proportion of the number of coarse fibers, the experimental group was myelin thickening, the appearance of axonal integrity.It was no significant among bundles of connective tissue proliferation, blood vessel wall was thinner among nerve fibers, endogenous cells without swelling, luminal patency, rich blood supply, nerve fibers gradually mature. Myelinated fibers in the control group were increased significantly in the number, but there were no significant increases in the proportion of coarse fibers, myelin previous thickened connective tissue among the beam reduction, vascular wall thickness among nerve fibers, endothelial cell swelling.3.2 the change situation of gastrocnemius motor endplate(MEP) after modeling One week the experimental group MEP stained brown, oval, slightly lighter color, endplate edge myocyte edema. The changes of control group were similar to experimental groups. At second weeks, the experiment group MEP staining shallow, uneven, different forms; the control group appeared stain section blank area, the number of rare endplate.At fourth weeks, the experiment group MEP gross forms, identifiable by light microscopy to further reduce the number; the control group MEP forms confusion, scarce. At eighth weeks, the two groups MEP near normal gross morphology, stained uniform, the two groups had no significant changes.4 electron microscope4.1 nerve entrapment at the electron microscope one week after modeling experimental group, myelin deformed severely,lamellar structure disordered marrow cord narrows mitochondrial inner cable disappear, showing a small amount of actin filaments. The changes of control group were similar to experimental groups. At 2 weeks, in the experimental group when compared with the previous myelin thickening, visible mitochondria, actin, microtubules, but a smaller number, sparse arrangement. Group myelin swelling of mitochondria cavitation necrosis, disintegration.At 4 weeks, the experimental group had significantly myelin hyperplasia, irregular arrangement, the mitochondria could be observed. Group myelin thickening deformation, narrowing axonal mitochondria disappeared, showing a small amount of actin filaments.At 8 weeks,in the experimental group increased regeneration of myelinated nerve fibers, myelin thickening arrangement rules, clear lamellar structure, showing mitochondria, actin increase compared with the previous, less Schwann cells. The control group was significantly thicker myelin, less irregular, mitochondria, actin, no Schwann cells.4.2 gastrocnemius motor end plate electron microscopy one week after modeling the synaptic gap widened during the primary and secondary folds shallow folds, mitochondrial swelling, changes in the experimental group and the control group had no significant differences. Two weeks of the experiment group MEP atrophy endplate postsynaptic region widens, primary and secondary folds shallow folds, synaptic gap widens, mitochondrial swelling, no degeneration; MEP had significant changes in the control group, the primary wrinkled twisted pleats and folds secondary mitochondrial degeneration.Four weeks of the experiment group MEP atrophy, primary and secondary folds distortions led to secondary synaptic gap widens, mitochondrial degeneration; regional simplify the control group MEP synapses, local secondary folds disappeared, synaptic the gap continued to widen. Eight weeks in the experimental group endplate normal synaptic gap, primary and secondary folds more obvious,can was telled. Control group was close to the experimental group.Conclusion: Qi Shen Huan Wu Capsules added scorpion, centipede and other blood in the soup through Bu Yang Huan Wu Meridians drugs reaching the blood circulation, Tongbi active role.Through this study, it can be concluded: Qi Shen Hhuan Wu Capsules can delay degenerative changes in muscle atrophy and motor endplate, can promote nerve repair and regeneration of the effect. Its mechanism of action is to improve nerve entrapment microenvironment and to promote Schwann cell proliferation and regeneration of nerves and blood vessels to promote growth and improve the blood supply, to reduce local inflammation.