| OBJECTIVE:To investigate the expression of NIK and IKKβ binding protein (NIBP), phosphorylated p65 (p-p65), zinc-finger E-box binding homeobox 1 (Zebl), Vimentin and E-cadherin levels in normal colon mucous membrane tissues, colon adenoma tissues and colorectal cancer tissues, and to analyze the relationship between the expression of NIK and IKKβ binding protein (NIBP) and epithelial-mesenchymal transition in human colorectal cancer.METHODS:The method of immunohistochemistry was used to detect the expression of NIBP, p-p65, Zeb1, Vimentin and E-cadherin in 114 paraffinembedded colorectal cancer tissues,20 colon adenoma tissues and 50 colon normal mucosa tissues. In addition, the level of serum TNF-a in the 184 patients were tested by method of ELISA.RESULTS:The level of serum TNF-a of colorectal cancer pantient with metastasis group and without metastasis group obviously higher than those in colon adenoma group and normal group[(34.46±14.81) pg/ml vs (29.26±12.96) pg/ml vs (18.47±8.63) pg/ml vs (16.81±7.97) pg/ml], (P<0.05). The positive expression of NIBP, p-p65, Zebl and Vimentin in colorectal cancer tissues with metastasis were much higher than those in tissues without metastasis, colon adenoma tissues and colon normal mucosa, whereas E-cadherin just the opposite, P<0.05. The positive expression of NIBP, p-p65, Zeb1, Vimentin and E-cadherin in colorectal cancer tissues was related with depth of invasion, TNM clinical stage, lymph node and distant metastasis (P<0.05). There are obvious correlation between NIBP, Zeb1 and p-p65 expression levels, E-cadherin, Vimentin and Zebl expression levels, E-cadherin, Vimentin and NIBP expression levels, P<0.05.CONCLUSION:NIBP is over expressed in colorectal cancer tissues, and may be related to the activation of nuclear factor-KB (NF-κB)signal pathway and the occurrence of EMT, and may play an important role in the development, invasion and metastasis of colorectal cancer. |
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