CD 10, BCL-6, MUM-1 And BCL-2 Expression In Diffuse Large B Cell Lymphoma And Its Relationship With Prognosis

Objective:To discuss CD10,BCL-6,MUM-l and BCL-2 expression in diffuse large B cell lymphoma and its relationship with prognosis.Methods:Collection of the affiliated tumor hospital of GuangXi Medical University from 2006 to August 2014 diagnosed cases of diffuse large B cell lymphoma, immunohistochemical include one of the CD10, BCL-6,MUM-1,BCL-2 indicators,Follow-up for all patients. Statistical analysis using SPSS 13.0 software, through the survival analysis, the relationship between its expression and prognosis were retrospectively analyzed, at the same time analysis the different immune phenotype, gender, age, level of lactate dehydrogenase, along with the B symptoms, clinical stage, and the number of organ involvement, international prognostic index, discusses its relationship with the prognosis of diffuse large B cell lymphoma.Overall survival (OS) calculated from diagnosis to patients with any reason to cause death, termination, or follow-up deadline is still surviving patients included in the truncation.Progression-free surial (PFS) calculated from diagnosis to tumor recurrence, progression or death.Results:(1) CD10, BCL-6, MUM-land BCL-2, positive rate was 30% (40/133),71.1% (91/128),77.1% (91/118),73.4% (69/94).Can be divided into type of DLBCL patients,47 patients with GCB subtype (39.5%),72 patients with non-GCB subtype (60.5%).(2) The single factor analysis showed that CD 10 positive PFS was better than negative (CD 10 positive and negative groups compare three years PFS were 55.0% vs 30.5%, p=0.025), the OS has no statistical significance (CD 10 positive and negative groups were compared for 3 years OS 82.9% vs 59.8% respectively,p=0.079), the BCL-6, MUM-land BCL-2 has no influence on prognosis (OS, PFS p values were greater than 0.05).(3) To other clinical feature lines of single factor analysis showed that elevated lactic acid dehydrogenase (Elevated LDH group compared with normal group,3 years OS 48.7% vs 83.3%, p=0.002;3 years PFS 21.3% vs 51.4%,p=0.000), IPI score 3 to 5 points (IPI score 0-2 group compared with 3-5 groups,3 years OS 76.7% vs 54.7%, p=0.025;3 years PFS 46.7% vs 25.5%, p=0.01) is associated with poor prognosis, â…¢-â…£ stage DLBCL has a worse PFS (3 years PFS vs 25.7% and 46.5%,p=0.02), the OS no statistical difference (3 years OS 60.5% vs 71.8%,p=0.07).(4) Multiariable COX model analysis, elevated lactate dehydrogenase, IPI are independent risk factors affect the prognosis of patients (P values are less than 0.05).Conclusion:(1)The single factor analysis showed that CD 10 positive, â…¢-â…£ period, elevated lactate dehydrogenase, IPI is the influencing factors of DLBCL patients with PFS.(2)The single factor analysis showed that elevated lactate dehydrogenase, IPI is the factors affecting the prognosis of DLBCL patients with OS.(3)Multiple factors analysis showed that lactate dehydrogenase levels, IPI is the independent risk factors affecting the prognosis of patients with DLBCL.