Study On The Relationship Of Th17 Cells And Aplastic Anemia

Background and purposeBackground:Currently, aplastic anemia (AA) is an autoimmune disease, whose pathogenesis is not clear, and the main pathogeny is relevant to abnormal immune function at present.Study shows that, in most cases, the specific autoimmune response of bone marrow hematopoietic stem cells mediated by T lymphocyte is the main pathological mechanism of aplastic anemia. Thl cell polarization of T cells in patients with aplastic anemia and the CD4+T cells play an important role in the regulation.T helper cells 17(Thl7) is a newly identified T cell subsets in recent years, which cells immune response has been confirmed very important in pathogenesis of autoimmune disease, whereas the relationship with AA is still unclear.Many related researches have been developed to understand the relationships among the function, mechanism and related cytokine of Thl7 cell, and the effect they played in AA. retinoid-related orphan nuclear receptor yt (ROR-ytmRNA) mRNA and cytokines Interleukin 17(IL-17)、Interleukin 23(IL-23) levels and Thl7 closely related function may reflect the situation Thl7 cells. Purpose:the experiment aims to discuss the relationship between Thl7 cells and mechanism of AA through studing the variety of Thl7 cells related cytokines like IL-17、IL-23 and the lever of ROR-ytmRNA in peripheral blood of patients with aplastic anemia after allogeneic hematopoietic stem cell transplantation.Research method30 patients with AA received allogeneic hematopoietic stem cellt ransplantation in Military General Hospital of Beijing PLA adopted in from September 2012 to July 2014, control group with the same period in the experimental group, correspondingly 30 normal cases from medical examination center in the same period in the control group.Using enzyme linked immunosorbent assay (EILSA) to detect the expression of IL-17 and IL-23.Testing the level of transcription factor RORγt(RORγtmRNA)in peripheral blood mononuclear cells via reverse transcription polymerase chain reaction (RT-PCR).Result1. Before transplantation, level of IL-17 and IL-23 in serum was (29.42±1.01) pg/ml, (193.14±9.58)pg/m respectively in group AA which significantly higher than the after transplantation AA group (20.61±2.33) pg/ml, (145.25±7.31) pg/ml and the normal control group (16.23±2.15) pg/ml, (128.57±11.13)pg/ml (P<0.05).The difference was statistically significance while the after transplantation AA group level compared with the normal control group, there was no significant difference (P> 0.05).2. The expression of IL-17, IL-23 in peripheral blood IL-23 in group AA and the counts of WBC, HGB, PLT which detected at the same period via the blood routine test showed a negative correlation (P<0.05), at the same time, there were no relevance between the level of IL-17, IL-23 and the counts of WBC, HGB, PLT in recovery group and normal control group (P>0.05)after transplantation.3. Before transplantation, the relationship between the level of IL-17, RORyt mRNA (6.549±0.528) before decreased was significantly higher than after transplantation group AA (3.741±0.282) and normal control group (2.637±0.375), whose difference had statistical significance (P<0.05). However group AA compared with the normal control group showed no significant difference (P>0.05)after transplantation.Conclusion(1)The expression lever of IL-17、IL-23:the expression level of AA patients with aplastic anemia was higher than the control group and AA group after transplantation, and decreased after allogeneic hematopoietic stem cell decreased, AA group before transplantation and IL-17, IL-23 with the same period showed a negative correlation between blood after transplantation AA group had no correlation with the same period in the blood, that revealed IL-17 and IL-23 played a certain role in the immunepathogenesis of aplastic anemia.(2)The expression of transcription factor RORγt in the peripheral blood of patients with AA increased and decreased after transplantation. (3)Th17 cells may serve as indicators and monitoring to assess the efficacy of treatment in AA patients with transplantation. (4) Through treatment for Th17 cells may provide a new treatment AA ideas.