Scutellaria is a commonly used Chinese herbal medicine, has antibacterial, antiviral activity, with a wide range of uses in the pharmaceutical, food, daily chemical industry. In this article, extracton of total flavonoids was compared with WREã€ERE〠GWREã€CERE and GERE. It is found that CERE and GERE were the optimum extraction methods for the extraction of flavonoids. The effects of temperature, ethanol concentration, extraction time, enzyme dosage and liquid to solid ratio on cellulose assisted ethanol extraction were investigated by single factor experiment. The results show that the temperature of 50℃,50% of the ethanol concentration, extraction time 6h, the dosage of cellulose 50U/g is the optimum conditions. In this condition, the yields of total flavonoids reached 19.85%, about 7.5% higher than single ethanol reflux extraction (ERE). The parameters of GERE were optimized by the single factor experiments, the optimal conditions were 1.1 mg GO/g dry raw material, ethanol 60% (v/v), ratio of liquid to solid 15(mL/g) at 70℃ for 6h. Under optimized conditions, the yields of total flavonoids and baicalein by GERE reached 24.95% and 0.38%, about 9.72% and 375% higher than ERE, respectively. SEM results revealed that after treated with GO, the radix scutellaria surface appeared cracks, ravines and small holes. The structural disruption of wall reduces the mass transfer resistance and accelerates the leaching of flavonoids by ethanol solvent. XRD and FTIR results showed that GO reduced the crystallinity and the grain sizes of the radix scutellaria cell, changed the internal structures of cellulose as well as cellulose accessibility, improved the hydrolysis efficiency. HPLC results revealed that the content of baicalein in extract by GERE was significantly higher than that by ERE, which means that GO can catalyze the hydrolysis of baicalin to baicalein. Therefore, GERE has great potential for the extraction of natural products.On the kinetics research of GERE, The effects of temperature, ethanol concentration, extraction time, GO dosage and liquid to solid ratio were investigated.The results showed that the extraction kinetics of GERE is agreed with the three-stage model by Fick’s second law, and the extraction process is mainly controlled by the internal diffusion. The added of GO, destroyed the cell walls, thereby reduced the diffusion resistance, which increased the mass transfer rate and the diffusion coefficien D’. The experiments also got the relationship between temperature and D’, for ERE, lnD’=In(A/m2)-2.45/T+2nlnR0ã€extracted activation energy Ea was 20.37kJ/mol, for GERE, lnD’=In(A/m2)-1.69/T+2nlnR0+201n (T-Tc)2〠extracted activation energy Ea was 13.2 kJ/mol,35.2% lower thanERE. |