Design Of Radiation Damage Detection System Based On A Microfluidic Chip

Evaluation of radiation damage is very important for the people’s production and life, especially in terms of disease prevention and surveillance. Typically, commercial flow cytometers and confocal are used to detect radiation damage. Because they both are expensive, bulky and complicated to operate, it is impossible to popularize in people’s daily life. This paper presents a miniaturized and high sensitive cytometer using a microfluidic chip for evaluating the radiation damage.The paper designs the detection system of radiation damage which can detect the radiation damage accurately, the specific includes:This paper defines the assessment parameters of radiation damage which contains the mean fluorescence intensity and the radiation ratio and presents the detection principle of radiation damage detection system; According to the principle it designs the portable device which includes fluorescence detection module, impedance pulse detection modules, power supply module, mechanical structure module and data acquisition and display module; A compact optical focusing system and a shift-phase differential amplifier circuit are designed to improve the sensitivity of the flourescence detection module which consists of the excitation source of LED and a photodetector to detect the mean fluorescence of irradiated cells,then the paper studies and designs these modules in detail except the data acquisition and display module; After the completion of each module the portable device can be assembled to finish the detection and analysis of radiation damage; The measured fluorescence intensity and radiation are demonstrated a good linear correlation with the radiation damage. The developed cytometer can evaluate quantitatively the radiation damage by the mean fluorescence intensity and radiation radio with a significantly smaller amount of blood samples than a commercial flow cytometer and the confocal.