Study On Prpnephrium Triphyllum-Rheum-Loaded Solid Lipid Nanoparticles As Carrier For Transdermal Drug Delivery

ObjectiveTo study a new dosage form in the base of the preparation of PrpnepHrium triphyllum in Zhongshan hospital of traditional Chinese medicine. The new dosage form which is based on the new transdermal drug nanometer carrier——solid lipid nanoparticles combines the PrpnepHrium triphyllum, the special herbs in south of the Five Ridges, and the Rheum with the reliable quality standards and apparent effect on anti-inflammation and analgesia. The study is aim to prepare a composite nano drug carrier which has high physical stability, little drug leakage, sustained release effect and even suitable for transdermal drug delivery. The new carrier is mainly for common skin diseases in Guangdong such as dermatitis eczema, sore carbuncle furuncle swollen, snake bites, erysipelas Chuangdu etc., in order to relieve pain of patients, at the same time, enlarge the range of clinical application of topical preparations of compound prescription of traditional Chinese medicine, promote the development and reform of hospital preparation dosage form.MethodsThe topic inclued tow parts:(1)The study on preparation method of PrpnepHrium triphyllum-Rheum-loaded solid lipid nanoparticles:This part includes research on pHarmaceutical properties and preparation process. First, triphyllin A and emodin from PrpnepHrium triphyllum and Rheum were selected as the marker components and a HPLC method for the determination of them was established to screen out the preparation method of solid lipid nanoparticles and determination of encapsulation efficiency method. On the basis of these preliminary work and the single factor investigation, using the orthogonal test, the preparation method was established.(2) The study on transdermal permeation law of PrpnepHrium triphyllum-Rheum-loaded solid lipid nanoparticles:Using improved Franz pond diffusion method in isolated rat skin as transdermal barrier with the method of HPLC, the rule of percutaneous permeation of riphyllum-Rheum-loaded solid lipid nanoparticles was investigated and then to calculate the cumulative transdermal amount and other pHarmacokinetic parameters such as delay and percutaneous rate constant. ResultsThrough the experimental research above, the active ingredient——triphyllin A and emodin has been successfully encapsulated in solid lipid nanoparticles.To establish a method for determination of contents of index components by high performance liquid chromatography, the solid lipid nanoparticles prepared in laboratory methods has been selected and a method for determination of the entrapment efficiency of solid lipid nanoparticles carrier has been established.To study on the evaluation of preliminary pHarmacology characteristic of the solid lipid nanoparticles carrier, show its particle size, and found its potential distribution is reasonable, and able to stable storage of a model drug, and reveal its drug release in vitro, characteristic of sustained release preparations.The in-vitro transdermal experiments further showed that the drug released gently, and transdermal delay was shorter, the cumulative penetration amount was larger to compare with other dosage forms such as creams, gels with significant sustained-release effect. ConclusionThe preparation process of PrpnepHrium triphyllum-Rheum-loaded solid lipid nanoparticles carrier is simple and feasible, stable property, and has the high encapsulation rate. To compare with the previous use of dosage forms such as ointment and hydrogel agent, the new dosage has sustained-release effect and long-lasting remission in patients with skin disease pain, targeted drug delivery, while providing a a new idea for the development of the Hospital of traditional Chinese medicine compound preparation for external use.