Quantification And Activities Of Ten Constituents Of Xanthoceras Sorbifolia Bunge

AIM:to investigate the bioactive constituents of Xanthoceras sorbifolia in terms of their amounts, antioxidant, DNA scission protection and a-glucosidase inhibitory activities. METHOD:Simultaneous quantification of 10 X. sorbifolia constituents was carreid out by establishing and using an ultra high performance liquid chromatography-quadrupole mass spectrometry method (UHPLC-MS). The antioxidant activities were evaluated by measuring DPPH radical scavenging and DNA scission protective activities. The a-glucosidase inhibitory ativities were investigated by using a-glucosidase from Bacillus Stearothermophilus and disaccharidases from mouse intestine. RESULTS:We found that the wood of X. sorbifolia was rich in phenolic compounds with the contents of catechin, epicatechin, myricetin and dihydromyricetin being 0.12-0.19,1.94-2.16,0.77-0.91 and 6.76-7.89 mg·g-1, respectively. These 4 contituents strongly scavenged DPPH radical (EC50 being 4.2,3.8 and 5.7μg·mL-1, respectively) and remarkably protected peroxyl radical-induced DNA strand scission (92.10 %,94.66%,75.44% and 89.95%of protection, respectively, at a concentration of 10μM). A dimeric flavan 3-ol, epigallocatechin-(4β→8, 2β→0-7)-epicatechin potently inhibited a-glucosidase with an IC50 value as low as 1.2/μg·mL-1. CONCLUSION:The established UHPLC-MS method could serve as a quality control tool for X. sorbifolia. The high contents of antioxidant and a-glucosidase inhibitory constituents in X. sorbifolia support its use as complementation of other therapeutic agents for metabolic syndrome, such as diabetes and hypertension.