| Objective: To summarize the invigorating kidney and promoting blood flow for therapy, external treatment with drugs for treatment of traumatic treatment of fracture theory, application of Promote the blood circulation and supplement the kidney function Method in traumatic fracture. While the establishment of rabbit fracture model for bone paste, to observe the expression of VEGF in the healing process of bone paste on rabbits fracture, to further explore the molecular mechanism of bone paste in promoting fracture healing of rabbits, and provide a theoretical basis for the treatment of traumatic fracture Promote the blood circulation and supplement the kidney function.Methods:(1) theoretical discussion: with " Promote the blood circulation and supplement the kidney function " and "traumatic fracture" as the theme words,retrieval of computer database, consulting ancient and modern literature, summarized in Promote the blood circulation and supplement the kidney function for therapy, with the drug for treatment, physical treatment of traumatic fracture theory.(2)experimental study of 60 adult rabbits, making the left radius about 3mm bone defect model successfully with a small steel, the experimental animal were randomly divided into 2 groups, namely the Jiegu ointment group(experimental group) and model group(control group), the experimental group with the bone paste, small splint bandage fixation; control group not treated with the same size, fixed bandage splint.In molding after third, 6, 10 W at the end of the experimental group and the control group were killed by air embolism animal of each 10, the bone defect as the center, at both ends of each bone specimens with 0.5cm, 15%EDTA-Na2 decalcification,paraffin section, for callus histology and immunohistochemical detection of VEGF.(3)statistical data: all test data were statistically analyzed with SPSS17.0 statistical software, using(±s), using two sample t test to compare the.Results:(1) theoretical discussion: Promote the blood circulation and supplement the kidney function for therapy, the drug as a treatment for traumatic treatment of fracture is commonly used method, significant clinical efficacy.(2) the BMD measurement :respectively in building w 3 w, 6 w, 10 at the end of the line of bone mineral density test, experimental group was better than control group, BMD values have significant difference(P ﹤0.01).(3) the histological examination: after modeling, the end of 3W,the experimental group were significant proliferation of osteoblasts, and the formation of trabecular bone, bone like tissue, the surface of fracture ends with the formation of granulation tissue, some periosteal cells in the wound, bone hyperplasia of fibroblast,proliferation of fibrous tissue, bone fracture end of reparative regeneration; the control group specimens fracture broken end also shows that the formation of granulation tissue, but the osteoblast is not obvious, fibrous tissue regeneration and repair the broken ends of relatively less. After modeling the end of 6W, the experimental group were visible fracture bone tissue gradually increased, and the broken end boundary is obvious, the broken end of the fiber connection and fibrous callus had obvious fracture repair, obvious hyperplasia, broken end of the fracture surface of new bone formation; the control group specimens showed the surface of fracture ends withfibrous callus formation, but callus number and the number of osteoblasts in experimental group is little, relatively few of cartilage. After modeling the end of 10 W,the experimental group showed broken ends of fracture with osseous callus, bone Liang Jian visible callus in angiogenesis, bone marrow recanalization; the control group also can see the fracture ends with bony callus formation, but trabecular bone callus in the angiogenesis relatively less the bone marrow cavity, basic recanalization,and cartilage components visible less.(4) immunohistochemical detection of VEGF:the experimental group and the control group of fracture in different periods of VEGF were positive. After modeling the end of 3W, the experimental group and the control group bone specimen broken end VEGF expression intensity and weaker, two group of MOD values were significantly different(P < 0.01); model after the end of 6W, the experimental group and the control group bone specimen broken end VEGF expression was significantly enhanced the 3W at the end of Ming, in vascular endothelial cells cells, osteoblast, bone soft had higher cell expression of chondrocytes, which was the highest, two group of MOD values were significantly different(P < 0.01); model after the end of 10 W, the experimental group and the control group bone specimen broken end VEGF expression compared with the 6W weekend weakened, but the two group MOD values significant differences(P < 0.01).Conclusion:(1) Promote the blood circulation and supplement the kidney function method is based on the pathogenesis of traumatic fracture of the focus of kidney deficiency and blood stasis and the establishment of the therapeutic principles,Promote the blood circulation and supplement the kidney function method is often used in the treatment of traumatic fracture treatment, can to drug therapies or drugs for treatment of external therapy used alone, also can be two kinds of treatment with drug therapies and the combined use of drug therapy, has an important position in traditional Chinese orthopedics.(2) drug therapy as the treatment of traumatic fracture measures, has a long history, simple operation, easy to use, low price, a significant effect, is commonly used in treatment of Chinese medicine treatment of traumatic fracture.(3) external application of herbal medicine for the treatment of traumatic fracture, can promote the expression of VEGF in the process of fracture healing, so as to promote fracture healing, shorten the time of fracture healing. Jiegu ointment in the treatment of traumatic fracture is mainly applicable to the early and middle. |
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