Effects Of HepG2 Tumor Vaccine Transfected HGM-CSF Gene Mediated By HA Nanoparticles Combined With Cisplatin

Objective: To investigate the treatment effects of Hep G2 tumor vaccine transfected h GM-CSF gene mediated by HA nanoparticles combined with different doses of cisplatin into liver cancer in order to provide evidence for the best solution for liver cancer treatment thus increase our hope and confidence to conqure human tumor.Methods: Establish Hu-PBL-SCID Hep G2 tumor-bearing mice. When the xeno grafted to over 100 mm3, mice were randomly divided into 4 groups, five rats each group:(1)control group(Saline);(2)the vaccine group(Hep G2 cells transfected with GM-CSF gene after 60 Co irradiated);(3)the vaccine after low dose of cisplatin chemotherapy group;(4)the vaccine after maximum tolerated dose of cisplatin chemotherapy group; Examined their spleens CTL activity and TGF-α、INF-γ level in the serum and measured the volumn, weights and tumor inhibit rates of each group when the mice were sacrificed after the final therapy.Results:(1)The CTL activity of the vaccine group, the LDM group and the MTD group had significant change compared with control group(P>0.05).The CTL activity of the LDM group was significantly higher than all the other 3 groups(P<0.05), while the CTL activity of the vaccine group increased less significantly. Compared with other groups, moreinfiltration of TGF-α, INF-γ in the vaccine after chemotherapy group and less side effects were observed.Conclusion: The active immunization of Hep G2 tumor vaccine transfected h GM-CSF gene mediated by HA nanoparticles combined with the chemotherapy of low-dose cisplatin to liver cancer has synergistic effect for hepatoma, which provides the experimental basis for cooperation of tumor immune treatment and chemotherapy.