| Cerebral palsy is a group of syndromes due to the persistent movement and developmental abnormality of the posture and the movement limitation caused by the non progressive damage of the fetus and the infant during the development.. Is one of the most common childhood disabling diseases. According to the epidemiological reports of children with cerebral palsy, the incidence rate is about 2.1 per thousand。Children accompanied by a variety of movement disorders, as well as perception, cognition, communication, and behavioral barriers, these multiple obstacle coexist situation will cause serious influence to the child growth and finally integrate into society. it is essential to recognize and understand the causes of these obstacles, the relationship between them and the impact of these obstacles on the movement disorders for the treatment of cerebral palsy and the implementation of rehabilitation programs.The spasm of cerebral palsy muscle is the main reason of the peripheral nerve and muscle damage. Spasticity is characterized by involuntary muscle activation, increase in resistance to passive stretch the muscles, causing joint passive movement difficult. The resistance of the passive activity in clinic is closely related to the movement disorder of the children with cerebral palsy. The intervention of cerebral palsy caused by cerebral palsy, and the intervention of peripheral nerve and skeletal muscle disease, there is no related research. Engsberg Ross explained that the development of the gross motor is not restricted by the spasm. This is consistent with the other two reports, the upper limb muscle strength difference compared with the CP spasm status for children / adolescents fine motor function more influence. The feeling of the passive movement of the limb in the spastic cerebral palsy is caused by the change of the passive and the active muscle tone.. This study has a strong challenge and important significance for the study of the activity of muscular spasm and the passive muscle structure of passive active muscle. In-depth study of this problem is for these disorders in children with CP rehabilitation and management is very important, because of botulinum toxins and heavy load of functional strength training these frequently used rehabilitation measures, enhance the muscle strength may also play a further increases in muscle tension.The persistent spasm status is the cause of the muscle damage in the primary nerve of cerebral palsy, and the main reason of the neuromuscular barrier in cerebral palsy is the main reason for the neuromuscular disorder. The study on spastic cerebral paralysis rats as the research object, on the microstructures of the peripheral nerve and skeletal muscle pathology change was observed by transmission electron microscope, combined with evaluation on motor function of rats, aims to reveal the scalp site medication injection on spastic cerebral paralysis therapeutic effect on the secondary skeletal muscle and peripheral nerve pathological changes, through the review of the literature involving both at home and abroad on cerebral palsy rats peripheral nerve and skeletal muscle lesions has not been observed. ObjectsThe ischemia and hypoxia caused by spastic cerebral palsy rats model as the research object, scalp site medication injection to the major intervention measures, through electron microscope observation of animal experiment of peripheral nerve and skeletal muscle pathology change and to evaluate the motor function to research study of scalp site medication injection on children with spastic cerebral palsy treatment role, in order for the clinical treatment provide the basis. Methods1.The bilateral common carotid artery dissection of 200 7-day-old SD rat pups and left carotid artery ligation, right carotid artery with arterial clip clipping 3h, clamping the artery into filled with N2: O2 volume fraction for 92:8 nitrogen oxygen mixed gas of self-made hypoxia box in hypoxia 2.5h, remove, a week after observation of screening out the successful fabrication of spastic cerebral palsy rats model 45.2.the rats with spastic cerebral palsy were randomly divided into 3 groups, 15 rats in each group, and 15 rats in the other randomly selected rats as the control group and the other rats were treated as the blank group. Followed by marking into blank group, control group, site physiological salt injection water group, site of vitamin B1B12 injection group; model is established on the 14 th day, the site of vitamin B1B12 injection group in animals in frontal and parietal lobe head subcutaneous injections of vitamin B1B12 injection solution about 2ml, every other day, a total of 7 times, the site and saline injection group received the same amount of physiological saline injection, injection method and frequency. Four groups were given a certain environmental stimulation.3.The treatment 2 weeks after the end of rats in each group generally, wilderness experiment, experimental slopes, beam balance test and measurement step distance long assessment, assessment at the end of dissection made soleus, tibial nerve produced electron microscope. Electron microscope was used to observe the muscle and peripheral nerve myelin sheath morphology and comparison lesions. Results1.Evaluation of motor function:(see Table 1) Compared the number of squares in the first minutes through the wilderness, sites of vitamin B1B12 injection group(12.47 ± 0.48) than the control group(6.41± 0.30), the difference was statistically significant(p=0, F=34.37), pull the testing time, point injection of vitamin B1B12 group(1.58±0.07) than the control group(3.47±0.09), the difference was statistically significant(p=0.001, F=9.38), balance beam test hind slippage times, sites of vitamin B1B12 injection group(0.59±0.23) than the control group(4.41±0.34), the difference was statistically significant(p=0.007, F=7.55), step long, site of vitamin B1B12 injection group(7.62 ±0.28) than the control group(4.15±0.27), the difference was statistically significant(p<0.014, F=16.35).2.Electron microscopy to observe the structure of the peripheral nerve and the structure of Schwann cells.(see Figure 1, figure 2):The shape of myelin in the vitamin B1B12 group was injected with the myelin shape rule, the arrangement of the plate layer was compact and free of dissolution. The structure of Schwann cells was complete, and the mitochondria and endoplasmic reticulum were dissolved.. The control group was proliferated in myelin, irregular in shape, loose solution in lamellar, fractured in delamination, and in the layered structure, the size of the bubble structure was different.. Schwann cells within the visible size ranging from vacuole like structures, most crests of mitochondria and partial film dissolution, vague, ridge of breakage and loss, and reduction of rough endoplasmic reticulum attached ribosome numbers.3.Electron microscopy to observe the structural changes of the cellular structure of the flounder muscle cells.(see Figure 3, figure 4):The structure of the muscle cells in the vitamin B1B12 group was complete and the muscle cells were arranged regularly, and there were a lot of mitochondria and glycogen in the two segment and muscle membrane of the muscle cells.. The muscle fibers in the control group were dissolved, the mitochondria dissolved in the muscle fibers, the size of the vacuole like structure varied, and the number of mitochondria and glycogen in the mitochondria was decreased obviously. Conclusion1.1Scalp site injection therapy can relieve the peripheral nerve lesion of cerebral palsy rats.2.Scalp site injection therapy can improve the skeletal muscle lesions in cerebral palsy rats.3.Scalp site injection therapy can promote the development of motor function in cerebral palsy rats. |
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