| Heterophylla heterophylla Department Caryophyllaceae perennial herbaceous perennial plant false chickweed leaves vary Pseudostellaria heterophylla (Miq.) Pax ex Pax et Hoffin. The dried roots. Sweet, bitter, flat; the spleen and lung; Spleen Qi, Sheng Jin lungs. Spleen Qi, Yin double up is one important way to treat Diabetes Chinese medicine, it is widely used Taizishen to prescription in the treatment of diabetes. Our previous studies with different molecular weight polysaccharide heterophylla can significantly reduce high fat, sugar and streptozotocin-induced type 2 diabetic rats fasting blood glucose, improve glucose tolerance and insulin resistance, in which the molecular weight range of about 50 to 210 KDa The hypoglycemic effect of polysaccharides Taizishen best, and can relieve the symptoms of hyperglycemia and hyperlipidemia, insulin resistance.But hypoglycemic effect and mechanism of this component is unclear, and in vitro whether hypoglycemic effect at home and abroad are not reported, so we use cell glucose transporter model, cell glucose consumption model, promoting islet cell secretion Insulin three model experiments to investigate the hypoglycemic effect characteristics. The polysaccharide component Taizishen basis can increase HepG2 cells consume glucose and high glucose culture medium, serum levels of 2%, the reaction time was 48h, under polysaccharide concentration of 400mg/L glucose consumption maximum conditions. PF40 heterophylla polysaccharide at various concentrations inhibited HepG2 cells, glucose transport, inhibition increased with increasing concentration. Show PF40 heterophylla polysaccharide can inhibit HepG2 cells excessive glucose uptake. Concentration of insulin secretion and polysaccharide was dependent heterophylla polysaccharide secretion maximum concentration 400 mg/L when insulin.Polysaccharide substance is one of the three life sciences, widely exist in medicine. In recent years, Chinese herbal hypoglycemic activity is very active, many experts and scholars through chemical and pharmacological study found that many traditional Chinese medicine polysaccharide oral hypoglycemic effects, such as after a number of Chinese herbal oral yam polysaccharide, Angelica polysaccharides were found to have significant hypoglycemic activity. Our group uses a combination of different DEAE cellulose column exclusion gel polysaccharides were isolated and purified to obtain homogeneous polysaccharide, uniform access to two main types of polysaccharide, dextran and heteropolysaccharide, we selected glucan PHP-G and miscellaneous polysaccharide PHP-H for the study. But classical biochemical theory is that after entering the body through the gastrointestinal tract polysaccharide hydrolysis into monosaccharides to absorb monosaccharides form, if so, to take additional polysaccharide sugar intake, will only increase the diabetes condition. In recent years, on the polysaccharide of "oral absorption," there are many speculate the mechanism, the difficulty has been the focus of research and debate, the bottleneck is a polysaccharide drug development. This topic vitro digestion model, heterophylla homogeneous polysaccharide after digestion after 2h, molecular significant changes did not occur in artificial gastric and intestinal fluid, described Taizishen homogeneous polysaccharide is stable in simulated artificial stomach, intestinal fluid.Caco-2 cell line derived from human colon adenocarcinoma cells in vitro and is easy to maintain stability. Cultured mature Caco-2 cells can form a single continuous layer of intestinal epithelial cells and similar, differentiated villus surface AP (apcial, luminal side) and the substrate surface BL (basolateral, intestinal wall side). Exist in the intestinal epithelium in various transport systems, metabolic enzymes in Caco-2 cells are expressed. It is worth mentioning that, Caco-2 cells were maintained the P-characterized glycoprotein (P-gp) high expression. This paper uses the Caco-2 cells were successfully established in vitro absorption model, transmembrance rcsistance reaches 400Ω·cm2 can be used for transport experiments.PHP-G polysaccharide and polysaccharide in PHP-H AP→BL transport volume increased with increasing concentrations of polysaccharide, the amount present in a concentration-dependent transport, transport of drugs is a preliminary indication of passive transport. At each concentration, the amount of polysaccharide AP→BL cumulative transport and the Papp BL→ AP relatively large, on the one hand, indicating that passive transport transport polysaccharide-based, it also shows that there may not be drug efflux, namely Taizishen homogeneous polysaccharide may not have been the role of efflux protein P-gp efflux in the absorption process. The apparent permeability coefficient greater than homogeneous polysaccharide Taizishen 1×10-6 cm/s, show heterophylla polysaccharide has good oral bioavailability. AP →BL Papp did not change significantly explain both homogeneous polysaccharide is not a substrate for P-gp after joining P-glycoprotein inhibitors verapamil.Chlorpromazine, within Nystatin and amiloride were interfering with cell-specific clathrin-mediated endocytic pathway within caveolin-mediated endocytosis and macropinocytosis way. PHP-H uptake of drug-treated cells polysaccharide polysaccharide in comparison with the control group did not differ, PHP-H polysaccharides may not be in the form of endocytosis into cells. Experimental data show that:For PHP-G-glucan polysaccharide chlorpromazine treated cells uptake least, so within PHP-G-glucan using mainly clathrin-mediated endocytic pathway into the Caco-2 cells. Clathrin-dependent endocytosis pathway is the transmembrane transport of macromolecules classical pathway, this pathway is dependent on clathrin-coated pits, which is present in all mammalian cells, capable of transporting such as proteins, lipids, growth factors, antibodies, and other nutrients. |
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