A Study Of The Protein Expression And Clinical Correlations Of FGFR1,FGFR3 And Pokemon In Bladder Cancer

Objective: In this study, we use the immunohistochemical methods to observe the expression of FGFR3, FGFR1 and Pokemon in different pathological types and stages of bladder cancer. Through the results, we analyze the relationship between the expression of FGFR3, FGFR1, Pokemon and the tumor pathology, clinical factors. Then, we investigate their roles in the occurrence and progress in bladder cancer, in order to set a theoretical theory for the molecular treatment of bladder cancer.Methods: The expression of FGFR3、FGFR1 and Pokemon protein in 296 cases of bladder cancer tissues, 48 cases of carcinoma adjacent tissues was detected by the method of the PV9002 two-step immunohistochemistry. Then the proteins were compared by the combination of clinical stage, histological grade, incipient relapse, sex and age and other factors. We use the SPSS 18.0 statistical software for statistical analysis.Result: 1. The expression of FGFR1 protein is negative in carcinoma adjacent tissue, and it has positive results in UC tissue. The expression rate of Ta-T1 is 7.5%(16/213), and T2-T4 is 18.1% (15/83) at different clinical stages. The expression rate of low-level is 2.8% (2/72), low - high level is 11.9%(15/126) and high-level is 14.3% (14/98) at different pathological grade. The expression rate of FGFR1 gene has a positive relationship with clinical stage and histological grade in UC, and there is a statistically significant difference (P<0.05). The incipient expression rate is 6.8%(13/191), and the recurrence expression rate is 17.1%(18/105). The recurrence expression rate of FGFR1 is significantly higher than the incipient expression rate in the UC, and there is a statistically significant difference (P<0.05).2. The expression of FGFR3 protein is negative in carcinoma adjacent tissue, and it has positive results in UC tissue. The expression rate of Ta-T1 is 68.1% (145/213), and T2-T4 is 35.0%(29/83) at different clinical stages. The expression rate of low-level is 75.0%(54/72), low - high level is 60.3%(76/126) and high-level is 44.9%(44/98)at different pathological grade. The expression rate of FGFR3 gene has a negative relationship with clinical stage and histological grade in UC, and there is a statistically significant difference (P<0.05).3. The expression of POKEMON protein is negative in carcinoma adjacent tissue, and it has positive results in UC tissue. The expression rate of low-level is47.2%(34/72), low - high level is 66.7%(84/126) and high-level is 80.6%(79/98) at different pathological grade. The expression rate of POKEMON protein has a positive relationship with histological grade in UC, and there is a statistically significant difference (P<0.05). The incipient expression rate is 59.2%(113/191), and the recurrence expression rate is 80.0% (84/105). The recurrence expression rate of POKEMON is significantly higher than the incipient expression rate in the UC, and there is a statistically significant difference (P<0.05).Conclusions: 1.The expression of FGFR1 protein is associated with clinical stage, histological grade and recurrenceonset, and is not relevant to gender and age in UC. It may has an important relationship with the occurrence and the development in UC. 2. The expression of FGFR3 protein is associated with clinical stage and histological grade, and is not relevant to recurrenceonset, gender and age in UC. It may has an important relationship with the initial stages of the development of UC. 3. The expression of POKEMON protein is not relevant to pathological grading, recurrenceonset, clinical stage, gender and age in UC. It may has an important relationship with the occurrence and the development of UC.