Neuromyelitis Opticaastrocyte Damage And Neurological Disorders Relationship

Objective Explore the AQP4 -Ab GFAP, ApoE, IL-6, IL-10, TNF-a is associated with the pathogenesis of NMO, correlation with the severity of the patients with NMO function defect, and the relationship with the effect of glucocorticoid treatment.Methods Set up NMO group (n=30, in the acute stage 7 days) and the control group (n=28), with double antibody sandwich enzyme-linked Immunosorbent method (Enzyme-Linked Immunosorbent Assay, ELISA) to detect two groups of in CSF and serum AQP4 -Ab, GFAP, ApoE, IL-6, IL-10, TNF-a of the levels testing, comparing the difference between the two groups of data. Correlation coefficient of the association between CSF GFAP, ApoE, IL-6, IL-10, TNF-a of the levels and CSF AQP4-Ab level, while correlation coefficient of the association between CSF AQP4-Ab, GFAP, ApoE, IL-6, IL-10, TNF-a of the levels and the acute phase EDSS score of the NMO group. Detection of glucocorticoid various index changes in the serum of the NMO group before and after the intervention, and glucocorticoid treatment EDSS (Expanded Disability Status Scale) scores were compared before and after.Results The NMO group in CSF and serum AQP4 -Ab, GFAP, IL-6 levels were higher than the control group (P< 0.05), ApoE, IL-10 levels were lower than the control group (P< 0.05), The difference between TNF-a level and the control group was not statistically significant (P> 0.05). The NMO group in CSF AQP4 -Ab, IL-10 levels were lower than the serum (P< 0.05), CSF GFAP, ApoE, IL-6 is higher than the serum(P< 0.05). The CSF GFAP, IL-6 levels and the CSF AQP4-Ab level were positively correlated (P< 0.05), and the CSF ApoE, IL-10 levels were negatively correlated with CSF AQP4-Ab level(P< 0.05), the CSF TNF-a level associated with the CSF AQP4-Ab level was no significant (P> 0.05). The CSF AQP4-Ab, GFAP, IL-6 levels and the acute phase EDSS score were positively correlated (P< 0.05), the CSF ApoE, IL-10 levels were negatively correlated with the acute phase EDSS score (P< 0.05), the TNF-a associated with the acute phase EDSS score was no significant (P> 0.05). The NMO serum AQP4 -Ab, GFAP, ApoE, IL-6, IL-10, TNF-a levels showed no significant difference before and after the glucocorticoid treatment (P>0.05), the EDSS score after the treatment compared with pre-treatment was low (P< 0.05).Conclusion 1.The detection of the level of AQP4-Ab of NMO disease diagnosis and severity of disease have important indicative, provide the basis for clinical treatment of NMO.2. The acute phase of NMO GFAP, IL-6 can be increased, the CSF GFAP, IL-6 were related with the CSF AQP4-Ab and the NMO function defect degree, and they were predict likely to be involved in the pathogenesis of NMO, while they may provide theoretical basis of the observation of disease and drug treatment the NMO ’s curative effect judgment. 3. The acute phase of the NMO ApoE, IL-10 were reduced, the CSF ApoE, IL-10 were related with the CSF AQP4-Ab and the NMO function defect degree, and prompt the ApoE, IL-10 can be used to observe the disease prognosis, added exogenous ApoE, IL-10 may be used as a new starting point for the treatment of NMO.4. The NMO group EDSS score was lower after GC treatment, it can prompt hormone treatment of nerve protective effect.