Experimental Sduty On The Effect Of Wuyou Soup Decoction With Rat Depression Model On The Expression Of 5-HT1AR And 5-HT2AR

Objective:On rat isolated raise and Chronic mild stress stimulus unpredictability(Chronic unpredictable mild stress, CUMS) copy of depression model, observed Wuyou soup for solitary raise and Chronic stress depression model rats hippocampal CA1 area of serotonin receptor(5-hydroxytryptamine receptor, 5-HTR) the two subtypes of 5-HT1 AR and 5-HT2 AR expression, the effect of preliminary discussion on the viewpoints have soup if antidepressant mechanism with 5-HTR especially the expression of 5 HT1 AR and 5-HT2 AR, Wuyou soup treating depression provides foundations for the clinical application, in order to further study the antidepressant mechanism easy soup to provide experimental basis. Methods:1. The preparation of model: random will level 60 CL SD male rats were divided into 6 groups: blank control group, model group, Diane force Flupentixol and Melitracen Tablets group, safe dose in low dose group, easy soup soup group, Wuyou decoction high dose group, each group of ten only. Does not offer any stimulus, blank control group, the rest of the group with solitary keep chronic mild stress(CUMS) unpredictability boosted the depression in rats model, last 35 days. Before building the adaptive breeding in rats for a week.2. Grouping dosing: building after 35 days, giving medication. Blank control group, not to give free drinking water diet;Model group normal saline lavage,daily 2 ml each rat; Flupentixol and Melitracen Tablets group set of 9.5 mg/kg every day to;Wuyou soup(powder) low, medium and high dose group of lavage for every day: 0.41 g/kg,0.82 g/kg,1.64 g/kg.To 1 times a day for 35 days.3. Index detection:the rat behavior test before the experiment building(0 days),(35 days),after building(70 days)after treatment.On the 70 th day after the experiment with hematoxylin and eosin stain(HE staining) to observe the rat hippocampal CA1 area of positive changes in cell morphology and cell number; Observed by immunohisto chemical method, Wuyou soup of rat hippocampal CA1 area 5-HT1 AR and 5- HT2 AR expression effect. Results:1. Between groups of rats in feeding adaptability during a week, usually appear normal, behavior detection of no statistical significance(P > 0.05).2. After 35 days after building, compared with the blank control group, model group and the medication group rats normally change is bigger, the behavioral indexes significantly decreased(P < 0.05), suggesting building success.3. After 35 days after drug treatment, Flupentixol and Melitracen Tablets group compared with model group, Flupentixol and Melitracen Tablets group and one soup, behavioral indicators of high dose group increased significantly(P < 0.05).4. Compared with the blank control group, model group rats hippocampal 5-HT1 AR positive expression rate reduced(P < 0.05), 5-HT2 AR positive expression rate increased(P < 0.05); Flupentixol and Melitracen Tablets group compared with model group, Flupentixol and Melitracen Tablets force and one soup, high dose group of rats hippocampal 5-HT1 AR positive expression increased(P < 0.05), 5-HT2 AR positive expression reduced(P < 0.05). Conclusions:1. Solitary and chronic mild stress stimulus unpredictability can more successful preparation of ideal depression model rats, in order to further study the antidepressant mechanism of easy soup experimental basis.2. Wuyou soup can obviously increase the weight of depression model rats growth, food consumption, sugar, water consumption, open box experiment horizontal and vertical scoring the behavioral indexes, statistically significant difference(P < 0.05); At the same time can be significantly reduced in the rat forced swimming time, statistically significant difference(P < 0.05). Tip can effectively improve depression rats of depressive state.3. Immunohistochemical results showed that the rat hippocampal CA1 area there are 5-HT1 AR and 5-HT2 AR positive cells4. Wuyou soup have antidepressant effect, the antidepressant mechanism may be related to improve depression model rats hippocampal CA1 area cut 5-HT1 AR and 5-HT2 AR expression.