The Experimental Research On The Influence Of The Yupingfengsanjiaweifang On The Airway Reconstruction Of Asthma Mice

Objective This topic selection of TCM clinical treatment of asthma in yunnan effective prescription, yupinfeng flavored, application of the party intervention bronchial asthma airway remodeling in mice lesions, detection model in mice after use yupinfeng flavored party associated with airway remodeling in the lung tissue and serum cytokines(VEGF, MMP- 9 and TIMP- 1 and TGF- beta 1, etc.) the change of the expression, in order to preliminary clarify yupinfeng flavored party slow bronchial asthma airway remodeling may be the mechanism of action, the clinical application of the party yupinfeng flavored to provide theoretical support.Methods Establish a chicken ovalbumin(OVA) induced bronchial asthma model in mice, using yupinfeng flavored to intervene. A. 1 according to the principle of random participants mice were randomly divided into 6 groups, each group of eight, asthma were divided into normal control group, model group, positive drug control, yupinfeng flavored party intervention in high dose group, middle dose group, low dose group; A. 2 based on the method of OVA induced airway remodeling of asthma mice model;.through three pathological lung tissue of mice HE staining groups of mice were observed under optical microscope pathology score, lung tissue structure and to measure average lining alveoli interval(MLI); A. 4 enzyme-linked immunosorbent assay(ELISA) to detect the mice serum VEGF, MMP- 9 and TIMP- 1 and TGF- the content of beta 1, and compare the ratio of MMP- 9 / TIMP- 1. Results 1, the model group(group B) in mice after each OVA atomized excitation are: shortness of breath, sneezing, weeks of cyanosis, restlessness, nod breathing and tired v, etc.; The normal control group(group A) had no performance. Pathological model group(group B) mice showed bronchial and alveolar structure is clear, airway wall there is A lot of inflammatory cells infiltrating the pathological change, the normal control group(group A) mice organization bronchial and alveolar structure is clear, only A small amount of inflammatory cells infiltration, suggests building success. 2, yupinfeng flavored pathological intervention of asthma mice showed lung tissue inflammation cells infiltration, the lung tissue structure change and thickening of alveolar space interval expansion, such as pathology, intervention group compared with model group, pathological airway epithelium goblet cell hyperplasia in mice and thickening of alveolar space interval expansion, degree is not obvious. 3, yupinfeng flavored party groups of related cytokines in serum of mice(VEGF, MMP- 9 and TIMP- 1 and TGF- beta 1) levels of mice were lower in the model group, the significant difference(P < 0.01). Conclusion 1. Yupinfeng flavored can obviously relieve asthma airway inflammation in mice, among them, the dose group relieve airway inflammation effect is more obvious. 2. Yupinfeng flavored reduce asthma mice serum fibrosis factor TGF- beta 1, the secretion of VEGF, regulating the secretion of MMP- 9 / TIMP- 1 balance. 3, yupinfeng flavored to asthma airway inflammation, airway remodeling has certain regulation, the regulating mechanism may be through the inhibition of TGF- beta 1 and the expression of VEGF, regulating the secretion of MMP- 9 / TIMP- 1 balance.