| Objective1 In the present study, we established an ICH model by injecting type â…¦ bacterial collagenase (0.5 U) into the central striatum of male Sprague-Dawley rats.Objective1 In the present study, we established an ICH model by injecting type â…¦ bacterial collagenase (0.5 U) into the central striatum of male Sprague-Dawley rats.2 Our study showed that quercetin treatment offered beneficial effects in many aspects of ICH-related brain damage, including overall neurological function. Quercetin is a potent anti-inflammatory and anti-apoptosis agent and may prove to be a potential target for research and development of drugs for clinical treatment of ICH and ICH related brain damages.Objective1 In the present study, we established an ICH model by injecting type â…¦ bacterial collagenase (0.5 U) into the central striatum of male Sprague-Dawley rats.2 Our study showed that quercetin treatment offered beneficial effects in many aspects of ICH-related brain damage, including overall neurological function. Quercetin is a potent anti-inflammatory and anti-apoptosis agent and may prove to be a potential target for research and development of drugs for clinical treatment of ICH and ICH related brain damages.Method1 The animals were randomized to four groups:sham-operation group; ICH+vehicle group; ICH+5 mg/kg quercetin group; and ICH+50 mg/kg quercetin group.Objective1 In the present study, we established an ICH model by injecting type â…¦ bacterial collagenase (0.5 U) into the central striatum of male Sprague-Dawley rats.2 Our study showed that quercetin treatment offered beneficial effects in many aspects of ICH-related brain damage, including overall neurological function. Quercetin is a potent anti-inflammatory and anti-apoptosis agent and may prove to be a potential target for research and development of drugs for clinical treatment of ICH and ICH related brain damages.Method1 The animals were randomized to four groups:sham-operation group; ICH+vehicle group; ICH+5 mg/kg quercetin group; and ICH+50 mg/kg quercetin group.2 ICH+5 mg/kg quercetin group, where 5 mg/kg quercetin in 1 ml vehicle was administered i.p. to each animal 12 h after ICH; and (4) ICH+50 mg/kg quercetin group, where 50 mg/kg quercetin in 1 ml vehicle was administered i.p. to each animal 12 h after ICH. The animals in all four groups received an equivalent volume (1 ml) of the vehicle or quercetin in traperitoneal injection at a 12 h interval for 3 days.Objective1 In the present study, we established an ICH model by injecting type â…¦ bacterial collagenase (0.5 U) into the central striatum of male Sprague-Dawley rats.2 Our study showed that quercetin treatment offered beneficial effects in many aspects of ICH-related brain damage, including overall neurological function. Quercetin is a potent anti-inflammatory and anti-apoptosis agent and may prove to be a potential target for research and development of drugs for clinical treatment of ICH and ICH related brain damages.Method1 The animals were randomized to four groups:sham-operation group; ICH+vehicle group; ICH+5 mg/kg quercetin group; and ICH+50 mg/kg quercetin group.2 ICH+5 mg/kg quercetin group, where 5 mg/kg quercetin in 1 ml vehicle was administered i.p. to each animal 12 h after ICH; and (4) ICH+50 mg/kg quercetin group, where 50 mg/kg quercetin in 1 ml vehicle was administered i.p. to each animal 12 h after ICH. The animals in all four groups received an equivalent volume (1 ml) of the vehicle or quercetin in traperitoneal injection at a 12 h interval for 3 days.3 In the first reference 12 h and 3 days cerebral hemorrhage after start of the experiment improved neurological function in rats (modified neurological severity score, mNSS) standards, praxiology score.3days rats in each group were sacrificed, hematoma volume in each group were observed in rat brain samples of cerebral hemorrhage, cerebral edema, inflammation factor expression and apoptosis.Objective1 In the present study, we established an ICH model by injecting type â…¦ bacterial collagenase (0.5 U) into the central striatum of male Sprague-Dawley rats.2 Our study showed that quercetin treatment offered beneficial effects in many aspects of ICH-related brain damage, including overall neurological function. Quercetin is a potent anti-inflammatory and anti-apoptosis agent and may prove to be a potential target for research and development of drugs for clinical treatment of ICH and ICH related brain damages.Method1 The animals were randomized to four groups:sham-operation group; ICH+vehicle group; ICH+5 mg/kg quercetin group; and ICH+50 mg/kg quercetin group.2 ICH+5 mg/kg quercetin group, where 5 mg/kg quercetin in 1 ml vehicle was administered i.p. to each animal 12 h after ICH; and (4) ICH+50 mg/kg quercetin group, where 50 mg/kg quercetin in 1 ml vehicle was administered i.p. to each animal 12 h after ICH. The animals in all four groups received an equivalent volume (1 ml) of the vehicle or quercetin in traperitoneal injection at a 12 h interval for 3 days.3 In the first reference 12 h and 3 days cerebral hemorrhage after start of the experiment improved neurological function in rats (modified neurological severity score, mNSS) standards, praxiology score.3days rats in each group were sacrificed, hematoma volume in each group were observed in rat brain samples of cerebral hemorrhage, cerebral edema, inflammation factor expression and apoptosis.4 Collagenase law making rat model of cerebral hemorrhage were randomly divided into three groups:sham group, cerebral hemorrhage and cerebral hemorrhage+quercetin 50mg/kg group. BrdU 50 mg/kg were administered i.p. to each animal 12 h after sham-operation or ICH,1 times/d. At 7 and 14 days observed using BrdU labeling of endogenous neural stem cell proliferation and differentiation conditions.5 Through the above experiment, further design-related specific mechanisms of quercetin on nerve repair after brain hemorrhage experimental observation.5 Through the above experiment, further design-related specific mechanisms of quercetin on nerve repair after brain hemorrhage experimental observation.Result1 In the first 12 h and 3days administered the rats were observed neurological deficit scores, compared with cerebral hemorrhage, cerebral hemorrhage+ quercetin 50mg/kg group of neurological symptoms in rats were significantly improved neurological function value significantly lower, the difference was statistically significant (P<0.05).5 Through the above experiment, further design-related specific mechanisms of quercetin on nerve repair after brain hemorrhage experimental observation.Result1 In the first 12 h and 3days administered the rats were observed neurological deficit scores, compared with cerebral hemorrhage, cerebral hemorrhage+ quercetin 50mg/kg group of neurological symptoms in rats were significantly improved neurological function value significantly lower, the difference was statistically significant (P<0.05).2 Comparison by wet and dry weight, compared with cerebral hemorrhage after cerebral hemorrhage+quercetin and 50mg/kg group can significantly reduce cerebral hemorrhage in brain tissue edema, the difference was statistically significant (P<0.05). Bleeding lesion volume detected group compared with cerebral hemorrhage, cerebral hemorrhage+quercetin and 50 mg/kg in rats significantly promote hemorrhage contraction, the difference was statistically significant (P<0.05).5 Through the above experiment, further design-related specific mechanisms of quercetin on nerve repair after brain hemorrhage experimental observation.Result1 In the first 12 h and 3days administered the rats were observed neurological deficit scores, compared with cerebral hemorrhage, cerebral hemorrhage+ quercetin 50mg/kg group of neurological symptoms in rats were significantly improved neurological function value significantly lower, the difference was statistically significant (P<0.05).2 Comparison by wet and dry weight, compared with cerebral hemorrhage after cerebral hemorrhage+quercetin and 50mg/kg group can significantly reduce cerebral hemorrhage in brain tissue edema, the difference was statistically significant (P<0.05). Bleeding lesion volume detected group compared with cerebral hemorrhage, cerebral hemorrhage+quercetin and 50 mg/kg in rats significantly promote hemorrhage contraction, the difference was statistically significant (P<0.05).3 Compared with cerebral hemorrhage, cerebral hemorrhage+quercetin 50mg/kg group were able to significantly reduce the expression of inflammatory factors, the difference was statistically significant (P<0.05); Tunel staining method showed significantly reduced the number of apoptotic cells, the difference was statistically significant (P<0.05); Western Blot results showed pro-apoptotic protein Bax and apoptotic protein caspase-3 protein expression was significantly lower, the difference was statistically significant (P<0.05); inhibition of apoptosis protein Bcl-2 protein expression was significantly increased, the difference was statistically significant (P <0.05); immunofluorescence staining showed that caspase-3 positive cells was significantly reduced, and the difference was statistically significant (P<0.05)4 Compared with cerebral hemorrhage, cerebral hemorrhage+quercetin 50mg/kg group were in the SVZ-derived neural stem cell proliferation in the region significantly, the number of newborn neural stem cells increased significantly, and to maintain a longer growth period, the difference was statistically significant (P<0.05).4 Compared with cerebral hemorrhage, cerebral hemorrhage+quercetin 50mg/kg group were in the SVZ-derived neural stem cell proliferation in the region significantly, the number of newborn neural stem cells increased significantly, and to maintain a longer growth period, the difference was statistically significant (P<0.05).ConclusionOur study showed that quercetin treatment offered beneficial effects in many aspects of ICH-related brain damage, including overall neurological function. Quercetin is a potent anti-inflammatory and anti-apoptosis agent and may promotion endogenous neural stem cells proliferation.Quercetin should prove to be a potential target for research and development of drugs for clinical treatment of ICH and ICH related brain damages. |
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