NM23-H2 Suppresses Invasion And Migration Of Gastric Cancer Cells Through Inhibiting Wnt/β-catenin Signaling Pathway

ObjectiveTo detect the expression of NM23-H2 in different gastric cancer cell lines. To build a NM23-H2 over-expression cell line with stable transfection.To study the effect of NM23-H2 over-expression on BGC-823 cells biological behaviors such as cell proliferation, invasion, migration and the adhesion ability. To explore the impact of NM23-H2 on the expression of E-cadherin and β-catenin. To investigate the influence of NM23-H2 on Wnt/β-catenin signaling pathway.MethodsWestern-Blotting was used to observe the expression of NM23-H2 in different differentiation of gastric cancer cells. The recombinant NM23-H2 plasmid and mock-vehicle were transfected into cancer cells by lipofectamine2000. G418 was used for screening the transfected NM23-H2 over-expressed and empty vector of cells.Western-Blotting, RT-PCR and immunofluorescence were used to detect the expression of NM23-H2 after transfection and identification of transfection efficiency. The trypan blue staining was used to detect the cells viability and the MTT method was used to detect the cells proliferation.Transwell Chambers assays were used to detect cells invasion and migration ability. Hoechst 33342 dyeing was used to detect the adherion ability between cells and cells and MTT method was used to detect the adherion ability between cells and extracellular matrix.The expression of E-cadherin and β-catenin were determined by western-blotting. Wnta, P-GSK3β, WISP1, CD44 and MMP-7 protein levels were detected by western-blotting.ResultsNM23-H2 expressed in different differentiation of gastric cancer cells.NM23-H2 could inhibit the proliferation of BGC-823 cells in vivo.NM23-H2 could inhibit the invasion and migration of BGC-823 cells in vivo.NM23-H2 regulated the adhesion of BGC-823 cells in vivo.NM23-H2 over-expression could inhibit Wnt/β-catenin signaling pathwayin vivo.ConclusionsNM23-H2 over-expression has no effect on cells viability, but it can inhibit the proliferation of BGC-823 cells.NM23-H2 over-expression can inhibit the invasion and migration of BGC-823 cells by increasing the expression of E-cadherin, reducing the expression of P-catenin, enhancing the adhesion between cells and cells, attenuating the adhesion between cells and extracellular matrix.NM23-H2 over-expression can inhibit proliferation, migration and invasion of BGC-823 cancer cells, enhance adhesion between cells, inhibit adhesion between cells and extracellular matrix through inhibiting Wnt/β-catenin signaling pathway.