Study Of Guben-jiannao Methods On Impact Dendritic Spines In The Hippocampus Of Model Rats And Cave0lin-1, P-tau

Objectives:Alzheimer’s disease(AD) as a refractory disease, its impact on the elderly population in the quality of life and even family and society is enormous. Therefore, to enhance the study of the pathogenesis of AD, the search for effective treatment and prevention of drug targets has important clinical value and practical significance. This study from the perspective of tau protein phosphorylation, discusses the mechanism and reinforce treatment of AD brain, providing experimental data and clinical evidence for medical treatment AD.Methods:60 Wistar rats, were randomly divided into six groups, namely the normal group, the control group, model group, Aricept group, lowdose group and reinforce brain Act(referred to as the low group),and reinforce the brain French high-dose group(referred to as the high group) 10. After adaptive feeding 1w, model group, Aricept group,the group of low, medium and high groups were performed stereotactic brain surgery bilateral hippocampal injection of A β1-42, complex preparation AD rat model, the control group was injected with saline control group not I deal with. Postoperative 3d, Aricept group, the low group, the high group were given the appropriate medication orally, blank control group, model group was given normal saline.After 4w, with Morris water maze tests of learning and memory ability;dendritic spines of neurons in the hippocampus morphology and density rat Golgi staining; Western Blot assay of rat hippocampus Caveolin-1,P-tau(Thr231, Ser396 sites) protein expression changed.Results:Behavioral results showed that: Compared with normal rats,both spatial learning and memory acquisition, or information storage capacity, learning and memory abilities of rats in the model group were reduced, there was a significant difference(P<0.01) data;compared with the model group each treatment group learning and memory abilities of rats models have improved indicators were statistically significant(P<0.01); and high brain consolidate law,low-dose group were significantly better than the Aricept group(P<0.01).Golgi staining test results show: the model number of hippocampal neurons in rats significantly reduced or even die, the cell gap increases significantly reduce the number of dendritic spines,arranged in confusion, compared with the normal group, the difference was significant(P<0.01). And after the intervention and reinforce the brain and formulas, its high and low dose groups of neurons and dendritic spine morphology significantly improved, a significant increase in the number and density, compared with the Aricept group,the difference was statistically significant(P <0.01).Western Blot test results show: the model rats hippocampus reduce Caveolin-1 expression, tau phosphorylation levels were significantly increased, with the normal group, the difference was significant(P<0.01); compared with the model group, the low group, high group can Caveolin-1 expression in different degrees, and tau phosphorylation levels drop, the difference was significant(P <0.01); compared with the Aricept group, more pronounced in the low group lower(P <0.01).Conlusions:Kidney-essence and marrow weak is senile dementia of thebasic pathogenesis. Guben-Jiannao methodes has solid days of this,brain puzzle, can effectively treat Alzheimer’s disease, showed significant improvement in learning and memory in AD rats and hippocampal neurons in dendritic spine morphology and density,significantly improved AD model large expression of Caveolin-1 rat hippocampus, inhibition of tau hyperphosphorylation.