Exosome-Formed MiR-150* Derived From Bone Mesenchymal Stem Cells Could Inhibit The Growth Of Glioma

Background: Exosomes are 30-120 nm membrane vesicles in diameter and 1.13-1.19g/m L in density, they can absorbed by recipient cells. Exosomes are secreted by many types of cells such as red blood cells, T cells, various of tumor cells and so on. Recently many evidences have showed exosomes can participate in the progression of multiple diseases via different mechanisms, and play vital role in biological process. Exosomes were initially considered as garbage bags for abandoned membrane parcels and molecular fragments. However bone mesenchymal stem cells derived exosomes as an effective treatment have gained more attention, because the properties of endogenous and shuttle between cells, exosomes from mesenchymal stem cells(MSC) are effective approach for nucleic-acid drugs delivery. Whether bone marrow mesenchymal stem cells(BMSCs) could secrete mi R-150*-enriched exosomes and inhibit glioma cells growth still remain unclear.Objective: Mi RNA-based therapeutics hold great promise for tumor suppression, this study was to investigate the effect of mi R150*-loaded exosomes on regulation of glioma cells proliferation and cell cycle.Methods: Quantitative real-time PCR on 15 glioblastoma tissues samples and normal controls were used to confirm the mi R-150* expression level. Western blotting analysis and electron microscopy were employed to test exosomal biomarkers and their morphology. Transfection assay were used to collect mi R150*-loaded exosomes from bone marrow mesenchymal stem cells(BMSCs) culture medium. CCK-8 and cell cycle assays were used to analyze mi R150*-loaded exosomes effects on glioma cells.Results: In this study, Level of mi R150* expression was much lower in glioblastoma than in normal tissues. Transfection assay successfully acquired mi R150*-loaded exosomes which derived from bone marrow mesenchymal stem cells(BMSCs). Furthermore, mi R150*-loaded exosomes could largely inhibited glioma cells proliferation and suppress cell cycle progression. Cell counting kit 8(CCK-8) assays also demonstrated mi R150* delivered in exosomes was much less toxic.Conclusions: this study demonstrated mi R150* is down-regulated in glioblastoma. Mi R150*-loaded exosomes could suppress glioma cells and exosomes may be a potentially efficient therapeutic delivery system..