Detection And Analysis Of Active Substance In Tumour Cell

Cancer, one of the most threatening diseases of the human being, incidence rate is becoming increasingly high as survival environment worsening. However, cure rate of cancer is very low. So it is a medical problem for early detection and treatment of the cancer. The earlier presence of cancer been found, the higher cure rate patient will have. Analysis and detection of active substance in tumour cells has a great significance for the prevention and treatment of cancer. This thesis reported new methods based on nanotechnology, DNA biosensor and fluorescence assay technology for detection and analysis of active substance in tumour cells. This thesis mainly consists of the following three parts:1、A new method was came up with for synthesis of silica mesoporous materials. By changing the amount of raw materials to adjust pore size on the surface of silica mesoporous materials. Adoption of special organics can realize organic functional modification, then the silica mesoporous materials physical and chemical properties will change on macro level. A new idea was created that the silica mesoporous can be used as nanobiocontainer.2、Base on fluorescence analysis technology and nanobiocontainer, a new way to detecting ATP was created. The complementary strand of aptamer was hooked up to the surface of nanobiocontainer. Then the stored probe was fixed on the surface of nanobiocontainer by DNA hybridization. As the result, the fluorescein was stored in the nanobiocontainer. When faced with ATP, the probe will leave off from container, the fluoresein will be released. By measuring fluorescence signal, we can realize quantitative analysis of ATP. At the same time, we make some desk study for nanobiocontainer used as drug delivery.3、A novel strategy was constructed to detecting of telomerase based on fluorescence detection technology and DNA loop amplifier technology. Firstly hairpin DNA was fixed magnetic bead, then aptamer of telomerase hybrid with part of hairpin DNA. Telomerase can break stem-loop structure of hairpin DNA, then the bioprobe catching lots of signal DNA and few of link DNA was added into the biochemistry reaction. By effect of polymerase, the probe on the hairpin DNA extended and the aptamer will leave off the hairpin DNA participating the next cycle, realizing amplification of signal. At the same time, lots of probes can be fixed on one single magnetic bead, lots of signal DNA can be fixed on one single AuNP, so this assay realized multiple amplification of the signal, having a high sensitivity for detection of telomerase.