The Effects Of Cognitive Impairment In Hippocampus Of Rats On Glucose And Lipid Metabolism As Well As Mechanisms

Objective To investigate the effects of cognitive impairment in hippocampus of rats on glucose and lipid metabolism as well as mechanisms, a series of experiments were established to provide experimental evidence for the study of the neural mechanisms regulating metabolism.Methods The Aβ1-42 oligomers were injected into the hippocampus with stereotactic frame to build cognitive impairment model of rats, the cognitive function of rats were detected by Morris water maze, automatic biochemical analyzer were used to detect glucose and lipid levels in rats, hippocampal neurons apoptosis in rats were done with TUNEL, the expression of AMPKαm RNA in liver and skeletal muscle was detected by RT-PCR, the expression of AMPKα and p-AMPKα in liver and skeletal muscle were detected by Western Blot.Results The findings of this study were as follows:1.The average time that the experimental group of rats climbed on the platform from respective quadrant into the water were(85.19±2.41)s in cognitive function test, significantly longer than the sham group(43.80±10.32)s and the control group(47.20±11.24)s(P<0.05); The Numberof experimental rats across platforms was 1.78±0.93 and the percentage of the rats staying in the original platform quadrant time was 18.43%±5.75%, also showed significant differences comparing with the sham group and the control group(P<0.05).2.The experimental group glucose and lipid levels, fasting plasma glucose(FPG) was(7.83±0.31) mmol/L; total triglyceride(TG) was(2.30±0.14)mmol/L; low-density lipoprotein(LDL) was(2.39±0.08) mmol/L and total cholesterol(TC) was(4.55±0.15) mmol/L, it was significantly higher than the sham group and the control group(P<0.05).3.The experimental group hippocampal neuronal apoptosis index was significantly increased with an average of 71.37%±3.15%, while the sham group and the control group were 18.66%±3.20% and 15.36%±4.25%,according to the statistically analysis and compareation, there was a significant difference(P<0.05).4.The expression of AMPKαm RNA in liver and skeletal muscle of the experimental group was 0.45±0.02 and 0.27±0.04, while significantly decreased Comparing with the control group and the sham group(P<0.05); the expression of AMPKα and p-AMPKα in liver and skeletal muscle of the experimental group was significantly lower than the sham group and the control group(P<0.05); the expression of AMPKαm RNA, AMPKα and p-AMPKα in liver and skeletal muscle of the sham group and the control group were not statistically significant.Conclusions The following inferences can be draw from this study:1.Aβ1-42 oligomers were injected in the hippocampus of rats which can prepare hippocampal cognitive impairment model of rats successfully.2.Glucose and lipid(FPG, TG, TC, LDL) levels increased in the rats withCognitive impairment, and hippocampal neuronal apoptosis increased,suggesting that cognitive impairment in rats with blood glucose and lipid levels increasing may be associated with the elevating of neuronal apoptosis in hippocampus.3.Cognitive impairment in hippocampus of rats can cause glucose and lipid levels elevating, which may be related to the increasing of hippocampal neuronal apoptosis in the rats with Cognitive impairment thereby affecting the expression of AMPKα in liver and skeletal muscle.