The Role Of MEG3-Mediated Generation And Development Of Pancreatic Carcinoma

Object: To explore the effect of long noncoding RNA MEG3 on pancreatic carcinoma, and to study its molecular mechanisms.Methods: The SW1990 human pancreatic carcinoma cells were infected by plasmid pc DNA3.0-MEG3, then, using real-time PCR to identified the gene’s expression in SW1990. The effect of MEG3 on proliferation was evaluated by MTT and colony formation assays. FACS was used for identifying apoptosis, Transwell assay with or without Matrigel basement membrane were used to evaluated the alteration of motility and invasion property. Western blot was applied to detect the expression change of p53 after MEG3 transfection in SW1990. The 5-aza-Cd R(5-aza-2-deoxy-cytidine) was used to explore the effect of DNA methylation on MEG3 expression.Results: Overexpression of MEG3 decreased SW1990 cells proliferation and induced apoptosis in vitro. However, motility and invasion property of SW1990 were not ever change by MEG3 overexpression. p53 protein levels were affected by MEG3over-expression in vitro. MEG3 expression was significantly increased in SW1990 cells treated by 5-aza-Cd R.Conclusions: Our findings indicate that MEG3 expression is lost in SW1990 cell that could be affected by DNA methylation. Moreover, MEG3 could regulate SW1990 cell proliferation and apoptosis, partially via the activition of p53.