The Growth-inhibitory And Enhanced Chemotherapy Sensitization Effects Of Recombinant ING4-Adenovirus On MCF-7 Breast Cancer Cells

Objective:To explore the growth-inhibitory effects on the MCF-7 human breast cancer cells of the adenovirus-mediated ING4 gene.Methods:The MCF-7 human breast cancer cells were infected with Ad ING4 and fluorescence microscope,RT-PCR and Western-Blot analysis were selected to detect the transcription of ING4 gene.Cell growth inhibition and apoptosis was detected by CCK-8 assay and flow cytometry.In addition,RT-PCR was selected to detect the expression of Bax and Bcl-2.Results:The cell-growth of MCF-7 cells infected by Ad ING4 was obviouslyinhibited,and the infected cells have a higher apoptosis rate.This phenomenon mayhave a relationship with the up-regulation of Bax and down-regulation of Bcl-2 and Survivin.Conclusion:Ad ING4 can inhibit the growth of MCF-7 cells and induce cellapoptosis,which may result in changing Bax and Bcl-2 expression.