Effects Of Sleep Deprivation On The Gene Expression Of Different Brain Regions Of Rats 5-Serotonin1A Receptor And Dopamine Receptor

BackgroundAvailable evidence suggests that, during the period of sleep deprivation and sobriety have different gene expression.Sleep deprivation for 5-HT, DA and other neurotransmitters change the existing related research, but the balun and regulatory mechanisms of synergy between them is unclear.ObjectiveTo analyze the effect of sleep deprivation model on the gene expression and to study the differences between various pathways of neurotransmitters involved in sleep regulation through the examination of the changing condition of the gene expressions of 5-HT1A, DiR, and D2Rin regions of hippocampus, hypothalamus and corpus striatum under different sleep deprivation models.Methods1.REM sleep deprivations were tested in 48 male rats of 10 weeks old for 24 hours,48 hours and 78 hours respectively.2.The incubation period, Level of scores, and the number of standing upright were tested through open field test to verify sleep deprivation models of the physical and mental fatigue.3.The gene expressions of the 5-HT1A, D2R, and D2R in regions of hippocampus, hypothalamus and corpus striatum were examined through RT-PCR technique.4.Statistical processing and analysis was carried out with SPSS 11.5. Statistic analysis was carried out to the measurement data with x±s. Comparison and analysis of the means between two groups was carried out with Independent-Sample test. Means among groups were compared with one-way analysis of variance. Pairwise comparison was carried out with LSD test. Correlation analysis was carried with Pearson. The result P<0.05 represents the absolute differences of the statistical results.Results1.The open field test of SD rats was carried out after sleep deprivation. The crossing score and rearing score fluctuate greatly. The cognitive ability of rats falls obviously. Physical and mental fatigue appears. All these prove that sleep deprivation inhabits he mental activities of organisms.2.Sleep deprivation affects the gene expression of 5-HT1A in the regions of hippocampus (F=56.203, P=0.000) and corpus striatum (F=77.288, P=0.000). The difference is statistically significant. The expression quantity of rats under sleep deprivation in the regions of hippocampus and corpus striatum surpass the control group and the difference is statistically significant (P<0.05). The express quantity of the 72 hours group exceeds that of the 24 hours (P=0.005) and the 48 hours (P=0.010) in the region of hippocampus. The differences of each group in the region of corpus striatum are of statistical significance (P<0.05). There is no change of the gene expression of 5-HT1A of each group in the region of hypothalamus (P>0.05).3.Sleep deprivation affects the gene expression of D2 in regions of hippocampus (F=74.708, P=0.000), and corpus striatum (F=80.687, P=0.000) and the difference is statistically significant. The expression quantity of rats under sleep deprivation in the regions of hippocampus and corpus striatum is inferior to the control group and the difference is statistically significant (P<0.05). The gene expression quantity is lower in those under long-time deprivation. The differences of the gene expression quantity of each group in regions of hippocampus and corpus striatum are statistically significant. There is no change of the gene expression of D2R of each group in the region of hypothalamus (P>0.05).4.Correlation analysis shows that the gene expression of 5-HT1A and D2R of the control group is not related in all regions. There is a negative correlation between the expression of the two genes of 24 hours group in regions of hippocampus (r=-0.868, P=0.025) hypothalamus (r=-0.883,P=0.02) and corpus striatum (r=-0.841,P=0.036) the expression of the two genes of 48 hours group and 78 hours group is also a negative correlation respectively in regions of hippocampus (r=-0.881, P=0.02; r=-0.878, P=0.021),and corpus striatum (r=-0.735, P=0.006; r=-0.855, P=0.03).5.Sleep deprivation has no effect on the gene expression of D1R in regions of hippocampus, hypothalamus and corpus striatum (.P>0.05).ConclusionThe gene expression of 5-HT1A and D2R change greatly in regions of hippocampus and corpus striatum. The expression of 5-HT1Arises while that of the D2R falls and there is a negative correlation. The results show that the regulation of the 5-HT system and the dopamine system to the sleep rhythm is cooperative at the receptor level.