Study On Hypolipidemic And Antithrombotic Effects And Its Mechanism Of Polysaccharide Of Cydonia Oblonga Mill

Objective: To explore the effects of COM polysaccharide on the different thrombosis model,Using high fat diet to induced rats hyperlipidemia,to observe the effects of COM polysaccharide on the blood lipid and liver function 。 Methods:1) Purified COM polysaccharide by Sevage method and determination the content by Anthrone- sulfuric acid method 。 A study of the effects of COM polysaccharide on a mouse model of thrombosis by injecting collagen-adrenaline in the vein of the tail,the classical model of arteriovenous thrombosis in rats,Respectively to investigate COM polysaccharide on rat arteriovenous thrombus weight, blood coagulation function,fibrinolytic function,6-keto-PGF1α, TXB2, VWF, P-Slection content to explore the influence of its resisting thrombosis.2) In the experiment of COM polysaccharide coing fall hematic fat, feeding high fat diet induce hyperlipidemia model, record the weights of rats in different times,after the success of the induced, to fill the stomach for 30 days, A fully automated biochemical analyzer was used to measure the following indicators determination of serum TC, TG, HDL-C, LDL-C and other lipid levels and the variation in content of AST,ALT;Determination of GSH-PX, SOD and MDA in the liver, such as the change of antioxidant capacity, At the same time observed the liver pathological changes。 Results: 1)In the COM polysaccharide anti thrombosis experiment, compared with the control group, each dose group of COM polysaccharide can reduce the arteriovenous thrombosis weight. Effects of blood coagulation function COM polysaccharide high dose group could significantly extend the TT, reduced the content of D- D, prolonged APTT, rised the content of AT-III. PT, FIB, PC content did not change significantly; Effect of COM polysaccharide in high dose group on fibrinolytic functionwas significantly improve PLG, improve t-PA content; The effects of COM polysaccharide on platelet function, high dose group was significantly increased in 6- keto- PGF1 a content and reduce TXB2 content, reduce the content of VWF.2) In the COM polysaccharide cholesterol-lowering experiments, compared with the model group,COM polysaccharide in each dose group can significantly reduced serum TC, TG, LDL- C,MDA content, inhibit the activity of ALT, AST; raising HDL- C content and increase SOD and GSH-PX, Found at the same time it also can effectively improve the phenomenon such as hyperlipidemia rat liver steatosis. Conclusion: COM polysaccharide anti-thrombosis role is mainly to reduce the weight of thrombus formation, 6- keto-PGF1 a, reduced the content of TXB2, VWF;Fall hematic fat experiment can significantly reduce the blood fat, inhibit the activity of ALT, AST, increased the activity of antioxidant system,improve liver function.