An Experimental Study On Efficacy Of The Silver Ions Healing Biological Gel Dressing For Acute Radiation Skin Wound

Objective:Comparing The Silver Ions Healing Biological Gel Dressing and Biafine in the skin wound of SD rats with acute β radiation damage effect, by the gross examination、histological examination、 integrinβ1 and p63 changes,to investigate the efficacy of The silver ions healing biological gel dressing for acute radiation skin wound.Methods : 126 normal rats, weight( 200 ±15) g. From 100 centimeters to irradiated the bare skin of rats’ buttocks singly 12 minutes and 52 seconds with 6Me V high energy β-ray produced by a linear accelerator, the total absorbed dose was 35 Gy, to build animal models of acute Ⅱ°beta ray skin injury wound, divided into 3 groups randomly(blank group, control group and experimental group), 42 in each group. The blank group without any treatment. The control group treated with Biafine immediately, three times a day. The experimental group treated with The Silver Ions Healing Biological Gel Dressing immediately after the radiation, three times a day. To compare different period of the wound change for naked eyes observed. After irradiation 1d, 3d, 5d, 7d, 14 d, 21 d, 35 d, each group to took 6 rats’ skin tissue of the irradiated region, fixed and embedded to make the pathological section. To compare different period of the histological change of the wound for hematoxylin-eosin staining. To detected the surface markers integrinβ1 and p63 expression with Image-Pro Plus 6.0 in different times after the Immunohistochemical staining. All data were expressed as mean ± standard deviation( ±S), the results using SPSS 18.0 statistical software to One-way ANOVA, P < 0.05 was considered statistically significant.Results:1、Gross examination: Irradiation on that day, the skin of the irradiation have no obvious changes, Three days later after irradiation, Blank group irradiated area dry skin began peeling, local congestive erythema. The control group and the experimental group have Erythema, but no peeling, erythema lighter. Continue to observe that the control group was significantly deepen the wound and form a thick crust, after the scab off the wound gradually healed sites were contracture state, the surface is not smooth, dark skin color than normal, congestion and edema was obviously and sparse hair growth. The healing process in experimental and control group was similar to blank group, but the newborn epithelial tissue was relatively flat, the color was closed to normal tissue, and hair growth was better. 2、Histological examination: Compared with blank group, the epidermal cells swelling mild and fibrous tissue hyperplasia more quickly, epithelial cell hyperplasia is apparent, and the inflammation was lighter in experimental and control group. 3 、 Immunohistochemical examination: The brown granules expression of integrinβ1 and p63 positive cells could be found in three groups, there are many brown granules located in basal cell layer and around the hair follicles, the experimental group was the strongest. At the same time point, The IOD values of markers integrinβ1 and p63 in another two group has statistical significance for the blank group(One-way ANOVA、P <0.05), IOD of positive cells was no statistical significance between experimental group and control group(P >0.05). The express of integrinβ1 and p63 reached a peak in 14-21 days after irradiation, and the highest appeared in experimental group, and then gradually reduced.Conclusions:1、From 100 centimeters to irradiated the bare skin of rats’ buttocks singly 12 minutes and 52 seconds with 6MeV high energy electron beam produced by a linear accelerator, the total absorbed dose was 35 Gy, to build animal models of acute Ⅱ°beta ray skin injury wound. 2、The Silver more bio gel dressing and Biafine similar efficacy Both to reduce swelling and inflammation cells, promote the proliferation of epidermal stem cells, The extent to promote the synthesis of collagen fibers, to accelerate wound healing and reducing skin damage, Also it relatively easy to access than Biafine and reasonable price, it worth to be use in the radiation skin wound...