The Effect Of SAA Gene Silencing Expression On Proliferation Of CNE2 Cells

Objective: We try to study the effect of SAA gene silencing expression on proliferation of CNE2 cells, and explore the affection of SAA on BCL-2、Caspase-3 and proliferation in CNE2 cells. Methods: We transfect the pGPU6/GFP/Neo-SAA vector to CNE2 cells with liposome, which previously successfully constructed by the research group. Then according to the screening of G418 and the forming of clone, we contrast pGPU6/GFP/Neo-SAA-CNE2 cell lines. As the same time, we take pGPU6/GFP/Neo-CNE2 cell lines and CNE2 cells as a control. Culture those group cells in vitro. Cell cycle of these cells was analyzed by flow cytometry. The ability of cell proliferation was inspected by plate cloning-forming test. Inspecting the expression of BCL-2 、 Caspase-3 and NF-КB in these three cells by western blotting. Results: 1 、 Western blotting shows that expression of SAA protein is significantly decreased in pGPU6/GFP/Neo-SAA-CNE2 cell lines. 2、Flow cytometry shows that the number of pGPU6/GFP/Neo-SAA-CNE2 cell lines in split phase is reduced. 3、Plate cloning-forming test shows that the lever of proliferation in pGPU6/GFP/Neo-SAA-CNE2 cell lines is reduced. 4、Western blotting shows that the expression of BCL-2 increased is decreased, but the expression of Caspase-3 and NF-КB is increased. Conclusion: 1、The pGPU6/GFP/Neo-SAA-CNE2 cell lines with SAA gene silencing expression is successfully established. 2、SAA has the effect on promoting proliferation of CEN2 cells, and there is a down-regulation to expression of BCL-2, a up-regulation to expression of Caspase-3 and NF-КB.