The Effect And Mechanism Of Hemopexin In Liver Transplantation Immunity

Objective To explore the role of Hemopexin in liver transplantation immunity and the mechanism.Methods Measuring levels of Hemopexin from recipients according to the degree of acute rejection in rat after liver transplantation from Lewis to BN.Splenocytes derived from lewis rat were used as stimulator cells and those from BN were used as responder cells in MLR assays in addition with different doses of Hemopexin, and the supernatant was used for ELISA test,mRNA extracted from the cells were performed RT-PCR.Results The levels of hemopexin decrease with the exacerbation of the degree of acute rejection. Compared with the control group, cell proliferation rate of high dose group (Hemopxin 50ug/ml) decreases (P<0.05), IL-10 significantly decreases (P <0.05), IL-17 significantly increases (P<0.05), IFN-γ in the four intervention groups significantly increases (P<0.05). The transcription levels of IL-10, IL-17, IFN-y are consistent with the ELISA. The transcription levels of Mo-1, Ho-1, Fth, PCNA in intervention group increases compared with the control group(P<0.05).Conclusion Hemopexin could be used as a potential diagnosis and classification indicator of acute rejection in liver transplantation; hemopexin could reduce acute rejection through anti-inflammatory response in the earlier period of liver transplantation and in the latter period could exacerbate graft rejection by activating adaptive immune response and changing the balance of Thl and Th2 cells. The net effect of hemopexin to the acute rejection need to be further researched by in vivo studies.