Study On Analysis And Pharmacological Effects Of Estrogen In The Oviductus Ranae

Purpose: The type and content of hormones on the components of oviductus ranae, the method of content determination of estradiol to establish different areas of oviductus ranae, and compared with estradiol content in shrimp, fish and so common in food. To explore the oviductus ranae on body growth, hormone levels and gonadal hormone receptor expression effect.Method: Combined through the use of GC-MS method, the species containing hormone ingredients oviductus ranae and the relative content was determined in the. Using HPLC, the content of estradiol in different habitats of oviductus ranae and other common food and so on a series of samples were determined. Using female Kunming mice 24, were randomly divided into high dose group, low dose group and blank group. Group administered intragastrically oviductus ranae, blank group was lavaged with physiological saline. After the last administration, ovary, uterus and adrenal from mice in each group were weighed, and the tissue sections were pathologic morphology, serum hormone level. By using the Western method for the detection of Blot expression in mouse uterus, ovary and adrenal protein in FSHR, TβR I and TβR II.Result: After a preliminary analysis, hormonal composition of oviductus ranae mainly contains 0.33% androsterone acetate, 0.35% prostaglandin, 0.52% medrogestone, 23.94% cholesterol and 0.87% estradiol. Comparison of estradiol content in different areas of the oviductus ranae, the average content of estradiol from Antu oviductus ranae is 5.24 g/g, the average content of estradiol from Huadian oviductus ranae is 6.50 g/g, the average content of estradiol production in Wangqing oviductus ranae is 7.97 g/g, the average content of estradiol Tonghua production of Oviductus Ranae is 6.39 g/g, the average content of Wangqi oviductus ranae in the production of estradiol for 4.21 g/g, the average content of estradiol production of Fusong oil of Rana chensinensis oil in 7.65 g/g. Comparison of oviductus ranae and other common food content of estradiol, the average content of estradiol in shrimps for 2.02 g/g, the average content of estradiol in fish is 1.09 g/g, the average content of estradiol in the egg yolk is0.303g/g,theaveragecontentofestradiolinporkis0.934g/g,theaveragecontentoffemaleglycolmilkpowderis1.75g/g.comparedwiththeblankgroup,solventgrouphadnoobviouseffectoncellviability,meansthatthesolventdmsointheconcentrationof2.5cellshadnoeffecton%comparedwiththecontrolgroup,estradiol,oviductusranaehascertainpromotionofhumanbreastcancercellproliferationin48 hours,especiallyatlow2.5ng/mlconcentration,proliferationof0.25ng/mlwhenpromoting,butwithoutstatisticalsignificance(p>0.05),in72 hourstheoviductusranaeeffectonhumanbreastcancercellproliferationwasnotobvious.theoviductusranaeonfemalemicebodyweight,wetweightofuterus,ovaryandadrenalglandwetweightofwetweightwerenotsignificantlyaffected,thecontentsofe2andpinserumincreasedsignificantly(p<0.05),thecontentoffshincreasedsignificantly(p<0.01),thecontentoftincreasedsignificantly(p<0.001),nosignificantdifferencebetweenthecontentchangesofprlandlh.theoviductusranaehighdosetotheexpressionlevelofadrenaltissueproteindruggroupfshrincreased(p<0.05),theothergroupdidnotchangesignificantly.conclusion:thistestmethodisreliable,effectiveandstable,canbeusedforthedeterminationandanalysisofoviductusranaeandothercommonfoodestradiolcontent.thatlongtimetakinglargedosesofoviductusranae,toacertainextent,willcauseincreasedexpressioninthebodye2,t,fshandpchangesinhormonelevelsandfshrprotein,hassomeestrogenlikeaction.