Preparation And Properties Of SF/PLCL Blend Membrance And Hybrid Porous Scaffold

Silk fibroin(SF) as a biomarerial with good biocompatibility can be biodegradable.Natural silk fibroin has excellent mechanical properties, while regenerated silk fibroin is weak, especially in terms of toughness. Poly(L-lactic acid-co-ε-caprolactone)(PLCL) is a biomaterial which has excellent mechanical property and good biocompatibility. This paper,using blend method, prepared SF/PLCL blend membrance and studied the influence of microstructure, condensed state structure, mechanical properties, adheresion and proliferation of L929 cells. The effect of process parameters on pore structure, condensed state structure and mechanical properties of SF/PLCL hybrid porous scaffold was also investigated after the scaffold was preparaed through salt-leaching.Firstly, the SF/PLCL blend membrance was fabricated by casting with the ratio of0/100, 25/75, 50/50, 75/25 and 100/0 respectively. There was phase separation after SF and PLCL was blended. The main condensed state structure of silk fibroin was changed from Silk Ⅰ to Silk Ⅱ after treated with ethanol. SF component which in SF/PLCL blend membrance shrank seriously. The weight loss in 37 ℃ water of untreated silk fibroin membrance was 22.13% which droped to 1.33% after ethanol treated. As the ratio of SF component increased, the water-binding ability and Young’s modulus of treated blend membrance were enhanced, while the breaking elongation decreased. Compared with pure SF membrance, the blend membrance showed better toughness and cytocompatibility when the ratio of SF/PLCL was 25/75.Secondly, choosing scaffold with the ratio of SF/PLCL is 25/75 to be experimental group,choosing pure PLCL and pure SF scaffold to be control group, the SF/PLCL hybrid porous scaffold was fabricated by salt-leaching method. As the weight of Na Cl increased,the porosity went up and average pore size went down. As the weight of(SF+PLCL)increased, the porosity of scaffold went up and the average pore size of scaffold went down.The results indicated that SF/PLCL hybrid porous scaffold only showed slightly phaseseparation. Meanwhile, there is no new covalent bond or crystal structure. Compared with pure PLCL porous scaffold, SF/PLCL hybrid porous scaffold showed larger compressive modulus resulting to more stable pore structure, and showed better thermostability comparing pure SF porous scaffold.In this study, we successfully prepared SF/PLCL hybrid porous scaffold using blend and salt-leaching method. In this experiment system, we investigated the best parameters is4 g Na Cl with 0.1 g SF and 0.3 g PLCL. With this paramenters, the porous scaffold with1 mm of thichness, 75% of porosity and 133μm of average pore size was fabricated.