To Preliminary Study The Material Basis And Pharmacodynamics On Bile Reguritation Gastritis From Piper Longum Root Extract

Objective: To Piper longum of root carried on the preliminary research of material foundation and discussion Piper longum root of extracts on bile reflux gastritis(BRG)model rats and its mechanism of action. Methods: Study of Piper longum root after alcohol extraction with methylene chloride, ethyl acetate, n-butanol gradient extraction,the income ethyl acetate and n-butanol extracts by BRG pharmacodynamics screening in the rat model,Using the radioimmunassay, pathological histology and immunohistochemical technique, From the histologic change, gastrointestinal hormones and inflammation medium perspective Piper longum root active ingredients effect and the mechanism of action of preliminary for the treatment of BRG. Results: 1. By high performance liquid chromatography(HPLC) method for three group of Piper longum root content determination of piperine,measured 1g Piper longum root contains 0.23 mg of piperine. 2. Piper longum root optimization of ethanol extraction process: 70% ethanol concentration, solvent quantity for10 times, extraction times for 2 time, extracting time for4 h. 3.(1) Compared with model group, the Piper longum root in the ethyl acetate extract middle dose(250 mg﹒kg-1), low dose group(125mg﹒kg-1) and Piper longum root n-butyl alcohol extract high dose group(400 mg﹒kg-1) to model of BRG rats serum GAS have inhibition(P<0.05); Compared with model group,the Piper longum root ethyl acetate extract of low dose group(125 mg﹒kg-1) and Piper longum root n-butyl alcohol extract high(400 mg﹒kg-1), medium(125 mg﹒kg-1), low dose(125 mg﹒kg-1) group to model of BRG rats serum TNF-α has obvious inhibitory effect(P<0.01).(2)Under light microscopy to observe the gastric mucosa pathological tissue,compared with model group, thePiper longum root ethyl acetate extract of low dose group(125 mg ﹒ kg-1) and Piper longum root n-butyl alcohol extract high dose group(400 mg ﹒ kg-1) on gastric mucosal protective(P<0.05).(3)Using immunohistochemical technique,compared with model group,domperidone group、the Piper longum root ethyl acetate extract of low dose(125 mg﹒kg-1) group and Piper longum root n-butyl alcohol extract high dose group(400mg ﹒kg-1) group can reduce cancer gene expression product C-erb-B2 protein positive expression(P<0.05); Piper longum root n-butyl alcohol extract high dose group(400 mg﹒kg-1) group can decrease cancer gene expression product PCNA protein positive expression(P<0.05). Conclusion: Piper longum root ethyl acetate and n-butanol extracts on bile reflux gastritis rats model has certain protective effect to gastric mucosa,Piper longum root ethyl acetate extract of low dose group and high dose group of n-butanol alcohol extract of gastric mucosal protective effect is most obvious, Its mechanism may be through inhibiting the inflammatory medium GAS, TNF-α and cancer gene C-erb-B2, PCNA protein expression of gastric mucosal protection.